P4: ER TO GOLGI T & VSMC

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this project is to determine the role of altered ER-to-Golgi trafficking of angiotensin II type 1 receptor (AT1R) and beta 2 adrenergic receptor (AR) in primary cultures of neonatal rat ventricular myocytes and vascular smooth muscle cells. There are three specific aims. First aim is to define the role of Rab1 and other vesicular transport regulators (Sar1, ARF1, Rab2 and Rab6) in the endoplasmic reticulum-to-Golgi transport. Second aim is to determine if alterations in the ER-to-Golgi transport, as a consequence of manipulating Rab1 function, is involved in receptor signaling. Third Aim is to determine the effect of Rab1 on protein expression profile.