Abstract
In a patient with an unknown bleeding disorder and an IgM λ paraproteinemia, we demonstrated by thin-layer chromatography immunostaining and enzyme-linked immunosorbent assay that this protein specifically bound to a number of glycolipids and glycoproteins which have terminal β-galactosyl or β-glucosyl residues. Binding to galactosylceramide or glucosylceramide was inhibited by both galactosylceramide and glucosylceramide. From these studies, it is apparent that the M-protein recognized both β-galactosyl and β-glucosyl residues. This M-protein was also shown to prolong the partial thromboplastin time of normal plasma. Thus, this case represents an example of anti-carbohydrate specificity of an IgM M-protein in association with a spontaneous bleeding disorder.
Original language | English (US) |
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Pages (from-to) | 226-232 |
Number of pages | 7 |
Journal | Archives of Biochemistry and Biophysics |
Volume | 255 |
Issue number | 2 |
DOIs | |
State | Published - Jun 1987 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology