TY - JOUR
T1 - A mechanism-based in vitro anticancer drug screening approach for phenolic phytochemicals.
AU - Hsu, Stephen
AU - Yu, Fu Xin X.
AU - Huang, Qin
AU - Lewis, Jill
AU - Singh, Baldev
AU - Dickinson, Douglas
AU - Borke, James
AU - Sharawy, Mohamed
AU - Wataha, John
AU - Yamamoto, Tetsuya
AU - Osaki, Tokio
AU - Schuster, George
N1 - Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PY - 2003/10
Y1 - 2003/10
N2 - Plant-derived phenolic compounds, including polyphenols (e.g., tannins), flavonoids, and phenolic acids, have been under investigation for their anticancer therapeutic and chemoprevention properties. Recently, certain mechanisms underlying the differential effects of green tea polyphenols (GTPPs) on tumor versus normal cells have been determined. These suggest that GTPPs may simultaneously activate multiple pathways. However, existing screening methods are insufficient for the identification of agents that possess both a cytotoxic effect on tumor cells and a protective effect on normal cells. The current study describes the establishment of an in vitro survival/apoptosis testing system based on detecting these mechanisms by a double-fluorescence method. This system is able to screen potential chemopreventive or therapeutic agents from (but not limited to) plant-derived compounds based on the pathways differentially activated by the agents. Tumor cell death and normal cell survival are detected simultaneously, in a device that co-cultures normal human cells adjacent to human tumor cells.
AB - Plant-derived phenolic compounds, including polyphenols (e.g., tannins), flavonoids, and phenolic acids, have been under investigation for their anticancer therapeutic and chemoprevention properties. Recently, certain mechanisms underlying the differential effects of green tea polyphenols (GTPPs) on tumor versus normal cells have been determined. These suggest that GTPPs may simultaneously activate multiple pathways. However, existing screening methods are insufficient for the identification of agents that possess both a cytotoxic effect on tumor cells and a protective effect on normal cells. The current study describes the establishment of an in vitro survival/apoptosis testing system based on detecting these mechanisms by a double-fluorescence method. This system is able to screen potential chemopreventive or therapeutic agents from (but not limited to) plant-derived compounds based on the pathways differentially activated by the agents. Tumor cell death and normal cell survival are detected simultaneously, in a device that co-cultures normal human cells adjacent to human tumor cells.
UR - http://www.scopus.com/inward/record.url?scp=2542443640&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2542443640&partnerID=8YFLogxK
U2 - 10.1089/154065803770380968
DO - 10.1089/154065803770380968
M3 - Article
C2 - 15090233
AN - SCOPUS:2542443640
SN - 1540-658X
VL - 1
SP - 611
EP - 618
JO - Assay and Drug Development Technologies
JF - Assay and Drug Development Technologies
IS - 5
ER -