A method for the in vitro determination of feline corneal endothelial permeability

Mark L. Mcdermott; Mitchell A. Watsky; Dayle H. Geroski; Henry F. Edelhauser

doi:10.3109/02713689009003468

A method for the in vitro determination of feline corneal endothelial permeability

Mark L. Mcdermott
, Mitchell A. Watsky
, Dayle H. Geroski
, Henry F. Edelhauser

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A mounting block for in vitro perfusion of the cat cornea is described. Using this apparatus and the techniques of Araie, the permeability (P^ac) of the normal cat corneal endothelium to carboxyfluorscein was determined to be 2.5 plusmn; 0.2 × 10^-4 cm/min. To assess the sensitivity of this technique in determining changes in P^ac associated with alterations in endothelial, morphology, three cats underwent 2 successive unilateral, central, 10 mm diameter circular areas of endothelial debridement 6 weeks apart. Six weeks following the second wounding all 3 animals underwent morphometric analysis and P^ac determination. A trend toward an elevation in P^ac with extreme reductions in cell density was observed.

Original language	English (US)
Pages (from-to)	1129-1136
Number of pages	8
Journal	Current Eye Research
Volume	9
Issue number	12
DOIs	https://doi.org/10.3109/02713689009003468
State	Published - 1990
Externally published	Yes

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.3109/02713689009003468

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title = "A method for the in vitro determination of feline corneal endothelial permeability",

abstract = "A mounting block for in vitro perfusion of the cat cornea is described. Using this apparatus and the techniques of Araie, the permeability (Pac) of the normal cat corneal endothelium to carboxyfluorscein was determined to be 2.5 plusmn; 0.2 × 10-4 cm/min. To assess the sensitivity of this technique in determining changes in Pac associated with alterations in endothelial, morphology, three cats underwent 2 successive unilateral, central, 10 mm diameter circular areas of endothelial debridement 6 weeks apart. Six weeks following the second wounding all 3 animals underwent morphometric analysis and Pac determination. A trend toward an elevation in Pac with extreme reductions in cell density was observed.",

author = "Mcdermott, \{Mark L.\} and Watsky, \{Mitchell A.\} and Geroski, \{Dayle H.\} and Edelhauser, \{Henry F.\}",

note = "Funding Information: ACKNOWLEDGEMENTS Supported in part by an unrestricted grant from Research to Prevent Blindness, Inc., by Ophthalrnic Core Grant EY01931, and Vision Research Training Grant T32EY07016, EY05609,",

year = "1990",

doi = "10.3109/02713689009003468",

language = "English (US)",

volume = "9",

pages = "1129--1136",

journal = "Current Eye Research",

issn = "0271-3683",

publisher = "Taylor and Francis Ltd.",

number = "12",

}

TY - JOUR

T1 - A method for the in vitro determination of feline corneal endothelial permeability

AU - Mcdermott, Mark L.

AU - Watsky, Mitchell A.

AU - Geroski, Dayle H.

AU - Edelhauser, Henry F.

N1 - Funding Information: ACKNOWLEDGEMENTS Supported in part by an unrestricted grant from Research to Prevent Blindness, Inc., by Ophthalrnic Core Grant EY01931, and Vision Research Training Grant T32EY07016, EY05609,

PY - 1990

Y1 - 1990

N2 - A mounting block for in vitro perfusion of the cat cornea is described. Using this apparatus and the techniques of Araie, the permeability (Pac) of the normal cat corneal endothelium to carboxyfluorscein was determined to be 2.5 plusmn; 0.2 × 10-4 cm/min. To assess the sensitivity of this technique in determining changes in Pac associated with alterations in endothelial, morphology, three cats underwent 2 successive unilateral, central, 10 mm diameter circular areas of endothelial debridement 6 weeks apart. Six weeks following the second wounding all 3 animals underwent morphometric analysis and Pac determination. A trend toward an elevation in Pac with extreme reductions in cell density was observed.

AB - A mounting block for in vitro perfusion of the cat cornea is described. Using this apparatus and the techniques of Araie, the permeability (Pac) of the normal cat corneal endothelium to carboxyfluorscein was determined to be 2.5 plusmn; 0.2 × 10-4 cm/min. To assess the sensitivity of this technique in determining changes in Pac associated with alterations in endothelial, morphology, three cats underwent 2 successive unilateral, central, 10 mm diameter circular areas of endothelial debridement 6 weeks apart. Six weeks following the second wounding all 3 animals underwent morphometric analysis and Pac determination. A trend toward an elevation in Pac with extreme reductions in cell density was observed.

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DO - 10.3109/02713689009003468

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SP - 1129

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JO - Current Eye Research

JF - Current Eye Research

IS - 12

ER -

A method for the in vitro determination of feline corneal endothelial permeability

Abstract

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