TY - JOUR
T1 - A modification of the epidermal scarification model of herpes simplex virus infection to achieve a reproducible and uniform progression of disease
AU - Goel, Neena
AU - Docherty, John J.
AU - Fu, Ming Ming
AU - Zimmerman, Daniel H.
AU - Rosenthal, Kenneth S.
N1 - Funding Information:
This work was supported in part by Public Health Service research grant 2 R44 AI43107-02A1 FROM NIAID to DZ and KSR.
PY - 2002/12
Y1 - 2002/12
N2 - A slight modification in the method used to remove the top keratinized layer of skin in the epidermal scarification model of HSV infection results in an easier, less painful, more uniform and reproducible means of infection. The back of mice was depilated and the top skin layer was removed either by scratching with the side of a 26 guage needle, or by abrading with sand paper or a hand held motorized pedicure/manicure instrument. The virus was then applied on the scarified or abraded skin and the mice were observed for lesion development from day 3 to 10 post-infection. A uniform pattern of lesion development in terms of onset of lesions by day 3, progression to zosteriform by day 5 occurred for mice whose skin was abraded whereas variability in the time course, progression of symptoms and greater trauma occurred for mice whose skin was scratched with needle.
AB - A slight modification in the method used to remove the top keratinized layer of skin in the epidermal scarification model of HSV infection results in an easier, less painful, more uniform and reproducible means of infection. The back of mice was depilated and the top skin layer was removed either by scratching with the side of a 26 guage needle, or by abrading with sand paper or a hand held motorized pedicure/manicure instrument. The virus was then applied on the scarified or abraded skin and the mice were observed for lesion development from day 3 to 10 post-infection. A uniform pattern of lesion development in terms of onset of lesions by day 3, progression to zosteriform by day 5 occurred for mice whose skin was abraded whereas variability in the time course, progression of symptoms and greater trauma occurred for mice whose skin was scratched with needle.
KW - Abrasion
KW - Epidermal-scarification model
KW - HSV
KW - Lesions
KW - Zosteriform
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U2 - 10.1016/S0166-0934(02)00160-X
DO - 10.1016/S0166-0934(02)00160-X
M3 - Article
C2 - 12393145
AN - SCOPUS:0036883606
SN - 0166-0934
VL - 106
SP - 153
EP - 158
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 2
ER -