TY - JOUR
T1 - A region of SLBP outside the mRNA-processing domain is essential for deposition of histone mRNA into the Drosophila egg
AU - Potter-Birriel, Jennifer Michelle
AU - Gonsalvez, Graydon B.
AU - Marzluff, William F.
N1 - Funding Information:
This work was supported by National Institutes of Health grants R01GM58921 (W.F.M.), GM29832-41S1 (W.F.M. and J.M.P.-B). and R01GM100088 to G.B.G. J.M.P.-B. also received support from NIH grant R25GM055336. Deposited in PMC for release after 12 months.
Publisher Copyright:
© 2021 Company of Biologists Ltd. All rights reserved.
PY - 2021/2
Y1 - 2021/2
N2 - Replication-dependent histone mRNAs are the only cellular mRNAs that are not polyadenylated, ending in a stemloop instead of a polyA tail, and are normally regulated coordinately with DNA replication. Stemloop-binding protein (SLBP) binds the 3' end of histone mRNA, and is required for processing and translation. During Drosophila oogenesis, large amounts of histone mRNAs and proteins are deposited in the developing oocyte. The maternally deposited histone mRNA is synthesized in stage 10B oocytes after the nurse cells complete endoreduplication.We report that inwild-type stage 10B oocytes, the histone locus bodies (HLBs), formed on the histone genes, produce histone mRNAs in the absence of phosphorylation of Mxc, which is normally required for histone gene expression inS-phase cells. Two mutants of SLBP, one with reduced expression and another with a 10-amino-acid deletion, fail to deposit sufficient histone mRNA in the oocyte, and do not transcribe the histone genes in stage 10B. Mutations in a putative SLBP nuclear localization sequence overlapping the deletion phenocopy the deletion. We conclude that a high concentration of SLBP in the nucleus of stage 10B oocytes is essential for histone gene transcription.
AB - Replication-dependent histone mRNAs are the only cellular mRNAs that are not polyadenylated, ending in a stemloop instead of a polyA tail, and are normally regulated coordinately with DNA replication. Stemloop-binding protein (SLBP) binds the 3' end of histone mRNA, and is required for processing and translation. During Drosophila oogenesis, large amounts of histone mRNAs and proteins are deposited in the developing oocyte. The maternally deposited histone mRNA is synthesized in stage 10B oocytes after the nurse cells complete endoreduplication.We report that inwild-type stage 10B oocytes, the histone locus bodies (HLBs), formed on the histone genes, produce histone mRNAs in the absence of phosphorylation of Mxc, which is normally required for histone gene expression inS-phase cells. Two mutants of SLBP, one with reduced expression and another with a 10-amino-acid deletion, fail to deposit sufficient histone mRNA in the oocyte, and do not transcribe the histone genes in stage 10B. Mutations in a putative SLBP nuclear localization sequence overlapping the deletion phenocopy the deletion. We conclude that a high concentration of SLBP in the nucleus of stage 10B oocytes is essential for histone gene transcription.
KW - Drosophila oogenesis
KW - Histone locus body
KW - Histone mRNA
KW - Maternal mRNA
KW - SLBP
KW - Stemloop-binding protein
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U2 - 10.1242/jcs.251728
DO - 10.1242/jcs.251728
M3 - Article
C2 - 33408246
AN - SCOPUS:85101543171
SN - 0021-9533
VL - 134
JO - Journal of Cell Science
JF - Journal of Cell Science
IS - 3
ER -