TY - JOUR
T1 - A simple method to identify NET-positive cells in isolated glomeruli
AU - Romero, M.
AU - Mosquera, J.
AU - Rodríguez-Iturbe, B.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1997/1
Y1 - 1997/1
N2 - Background. Reactive oxygen radicals are probably involved in the pathogenesis of human and experimental models of renal disease, yet current methods are inadequate to quantify and identify the cells producing reactive oxygen radicals. Methods and results. We used the nitroblue tetrazolium reaction to determine superoxide anion production in glomerular cells in phorbol myristate-stimulated glomerular suspensions and in isolated glomeruli from rats with nephrotoxic nephritis, ureteral obstruction, and puromycin aminonucleoside nephrosis. We were also able to identify these nitroblue tetrazolium + cells using specific appropiate antibodies. When the technique was tested in conditions known to increase reactive oxygen radicals, as phorbol myristate-stimulated glomeruli and glomeruli from animals with nephrotoxic nephritis and ureteral obstruction, increased number of nitroblue tetrazolium + cells were found. These cells were identified as glomerular intrinsic cells (Thy-1 +) or infiltrating leukocytes (leukocyte common antigen+ or antineutrophil +). Conclusion. This method may be useful to determine cells participating in glomerular damage induced by reactive oxygen radicals.
AB - Background. Reactive oxygen radicals are probably involved in the pathogenesis of human and experimental models of renal disease, yet current methods are inadequate to quantify and identify the cells producing reactive oxygen radicals. Methods and results. We used the nitroblue tetrazolium reaction to determine superoxide anion production in glomerular cells in phorbol myristate-stimulated glomerular suspensions and in isolated glomeruli from rats with nephrotoxic nephritis, ureteral obstruction, and puromycin aminonucleoside nephrosis. We were also able to identify these nitroblue tetrazolium + cells using specific appropiate antibodies. When the technique was tested in conditions known to increase reactive oxygen radicals, as phorbol myristate-stimulated glomeruli and glomeruli from animals with nephrotoxic nephritis and ureteral obstruction, increased number of nitroblue tetrazolium + cells were found. These cells were identified as glomerular intrinsic cells (Thy-1 +) or infiltrating leukocytes (leukocyte common antigen+ or antineutrophil +). Conclusion. This method may be useful to determine cells participating in glomerular damage induced by reactive oxygen radicals.
KW - Glomerulus
KW - Nitroblue tetrazolium
KW - Reactive oxygen radicals
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U2 - 10.1093/ndt/12.1.174
DO - 10.1093/ndt/12.1.174
M3 - Article
C2 - 9027795
AN - SCOPUS:0031022294
SN - 0931-0509
VL - 12
SP - 174
EP - 179
JO - Nephrology Dialysis Transplantation
JF - Nephrology Dialysis Transplantation
IS - 1
ER -