TY - JOUR
T1 - A somatic cell hybrid map of human chromosome 13
AU - Washington, Sarah S.
AU - Bowcock, Anne M.
AU - Gerken, Steve
AU - Matsunami, Norisada
AU - Lesh, David
AU - Osborne-Lawrence, Sherri L.
AU - Cowell, John
AU - Ledbetter, David H.
AU - White, Raymond L.
AU - Chakravarti, Aravinda
PY - 1993
Y1 - 1993
N2 - We have constructed a chromosome 13 somatic cell hybrid map using seven cell lines: PGMEA6, a hybrid containing the entire chromosome 13, and six hybrids containing various deletions of chromosomes 13 (BARF7, PPF22, KBF11, KSF39, CF25, and CF27). We have mapped 80 markers that define 10 regions of chromosome 13 with respect to 10 breakpoints in the mapping panel; these regions range in size from 4 to 24 Mb, with an average size of 8 Mb. The 80 markers sublocalized on our mapping panel include 10 Alu-PCR clones, 6 of which were converted to sequence-tagged sites; 40 (CA)(n) repeat-containing clones, 27 of which are micro-satellite PCR markers; 8 (AAAG)(n) repeat- containing PCR markers, 1 two-allele PCR marker, 4 genes or expressed sequences, and 17 anonymous DNA probes. This low-resolution physical map can be used as a backbone map for more refined physical mapping using radiation hybrids or yeast artificial chromosomes.
AB - We have constructed a chromosome 13 somatic cell hybrid map using seven cell lines: PGMEA6, a hybrid containing the entire chromosome 13, and six hybrids containing various deletions of chromosomes 13 (BARF7, PPF22, KBF11, KSF39, CF25, and CF27). We have mapped 80 markers that define 10 regions of chromosome 13 with respect to 10 breakpoints in the mapping panel; these regions range in size from 4 to 24 Mb, with an average size of 8 Mb. The 80 markers sublocalized on our mapping panel include 10 Alu-PCR clones, 6 of which were converted to sequence-tagged sites; 40 (CA)(n) repeat-containing clones, 27 of which are micro-satellite PCR markers; 8 (AAAG)(n) repeat- containing PCR markers, 1 two-allele PCR marker, 4 genes or expressed sequences, and 17 anonymous DNA probes. This low-resolution physical map can be used as a backbone map for more refined physical mapping using radiation hybrids or yeast artificial chromosomes.
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U2 - 10.1016/S0888-7543(11)80004-2
DO - 10.1016/S0888-7543(11)80004-2
M3 - Article
C2 - 8307557
AN - SCOPUS:0027742086
SN - 0888-7543
VL - 18
SP - 486
EP - 495
JO - Genomics
JF - Genomics
IS - 3
ER -