Abstract
We recently described a complex population of spermatozoal coding RNAs that are delivered to the oocyte on fertilization. These are derived throughout spermatogenesis, representing a record of past events. Recently, evidence has been provided that micro-RNAs are present in testes, suggesting that they might also be carried in ejaculate spermatozoa. To directly test this hypothesis, a unique microarray system capable of directly identifying antisense RNAs and predicted transcripts was utilized. RNA isolated from the ejaculate spermatozoa of 6 normal fertile men was directly hybridized to sense oligonucleotide arrays containing 10000 elements. This revealed 68 shared RNAs, some of which are similar to those previously defined as micro-RNAs, whereas others were the antisense of previously in silico-predicted transcripts. The results and implications of this study are described in this communication.
Original language | English (US) |
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Pages (from-to) | 70-74 |
Number of pages | 5 |
Journal | Journal of Andrology |
Volume | 26 |
Issue number | 1 |
State | Published - Jan 2005 |
Externally published | Yes |
Keywords
- Antisense RNA
- Cloning
- Development
- Fertility
- Microarray
- Novel transcripts
- Spermatozoa
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Reproductive Medicine
- Endocrinology
- Urology