Accumulation of isogloboside and ganglio-N-tetraosyl ceramide having blood group B determinant in the hepatomas of female LEC rats

Toshio Ariga; Noriyuki Kasai; Ichiro Miyoshi; Masanaga Yamawaki; J. Neel Scarsdale; Robert K. Yu; Takeshi Kasama; Takao Taki

doi:10.1016/0005-2760(94)00186-3

Accumulation of isogloboside and ganglio-N-tetraosyl ceramide having blood group B determinant in the hepatomas of female LEC rats

Toshio Ariga
, Noriyuki Kasai
, Ichiro Miyoshi
, Masanaga Yamawaki
, J. Neel Scarsdale
, Robert K. Yu
, Takeshi Kasama
, Takao Taki

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We have studied the neutral glycolipid composition of spontaneous hepatomas in LEC female rats. Neutral lipid fractions were isolated and purified by column chromatographies on DEAE-Toyopearl 650(M) and Iatrobeads. The neutral glycolipid fraction contained 3.2 to 4.4 μg lipid-bound glucose (Glc) per mg protein, and consisted of isogloboside (iso-Gb4, 50.8% of total neutral glycolipids) and IV³Gal, IV²Fuc, GgOse₄Cer (asialo-BGM1, 13.5%) as the major neutral glycolipids and Gb3 and iso-Gb3 (9.2%), GlcCer (7.2%), LacCer (6.1%) as the other species. The structure of iso-Gb4 was elucidated by gas-liquid chromatography (GLC), permethylation study, liquid secondary ion (LSI) mass spectrometry, and nuclear Overhauser enhancement spectroscopy (NOESY) and that for asialo-BGM1 by GLC, LSI mass spectrometry, and high-performance thin-layer chromatography (HPTLC)-overlay method using anti-asialo-BGM1 antibody. Isogloboside and asialo-BGM1 which are found in negligible amounts in normal liver tissues may represent excellent markers for studying tumor metastasis and cellular adhesion.

Original language	English (US)
Pages (from-to)	257-266
Number of pages	10
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	1254
Issue number	3
DOIs	https://doi.org/10.1016/0005-2760(94)00186-3
State	Published - Feb 9 1995
Externally published	Yes

Keywords

Blood group B-active glycolipid
Hepatoma
Isogloboside
LEC rat
Neutral glycolipid

ASJC Scopus subject areas

Biophysics
Biochemistry
Endocrinology

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10.1016/0005-2760(94)00186-3

Cite this

@article{ac4757e08948446eb21878eb33f7d094,

title = "Accumulation of isogloboside and ganglio-N-tetraosyl ceramide having blood group B determinant in the hepatomas of female LEC rats",

abstract = "We have studied the neutral glycolipid composition of spontaneous hepatomas in LEC female rats. Neutral lipid fractions were isolated and purified by column chromatographies on DEAE-Toyopearl 650(M) and Iatrobeads. The neutral glycolipid fraction contained 3.2 to 4.4 μg lipid-bound glucose (Glc) per mg protein, and consisted of isogloboside (iso-Gb4, 50.8\% of total neutral glycolipids) and IV3Gal, IV2Fuc, GgOse4Cer (asialo-BGM1, 13.5\%) as the major neutral glycolipids and Gb3 and iso-Gb3 (9.2\%), GlcCer (7.2\%), LacCer (6.1\%) as the other species. The structure of iso-Gb4 was elucidated by gas-liquid chromatography (GLC), permethylation study, liquid secondary ion (LSI) mass spectrometry, and nuclear Overhauser enhancement spectroscopy (NOESY) and that for asialo-BGM1 by GLC, LSI mass spectrometry, and high-performance thin-layer chromatography (HPTLC)-overlay method using anti-asialo-BGM1 antibody. Isogloboside and asialo-BGM1 which are found in negligible amounts in normal liver tissues may represent excellent markers for studying tumor metastasis and cellular adhesion.",

keywords = "Blood group B-active glycolipid, Hepatoma, Isogloboside, LEC rat, Neutral glycolipid",

author = "Toshio Ariga and Noriyuki Kasai and Ichiro Miyoshi and Masanaga Yamawaki and Scarsdale, \{J. Neel\} and Yu, \{Robert K.\} and Takeshi Kasama and Takao Taki",

note = "Funding Information: The authors wish to thank Ms. Akiko Kamimura for her technical assistance. This study was supported in part by a grant-in-aid for scientific research from the Ministry of Education Science and Culture of Japan (to N.K. and T.T.), and by a grant from the USPHS (NS-11853 to R.K.Y.).",

year = "1995",

month = feb,

day = "9",

doi = "10.1016/0005-2760(94)00186-3",

language = "English (US)",

volume = "1254",

pages = "257--266",

journal = "Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism",

issn = "0005-2760",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Accumulation of isogloboside and ganglio-N-tetraosyl ceramide having blood group B determinant in the hepatomas of female LEC rats

AU - Ariga, Toshio

AU - Kasai, Noriyuki

AU - Miyoshi, Ichiro

AU - Yamawaki, Masanaga

AU - Scarsdale, J. Neel

AU - Yu, Robert K.

AU - Kasama, Takeshi

AU - Taki, Takao

N1 - Funding Information: The authors wish to thank Ms. Akiko Kamimura for her technical assistance. This study was supported in part by a grant-in-aid for scientific research from the Ministry of Education Science and Culture of Japan (to N.K. and T.T.), and by a grant from the USPHS (NS-11853 to R.K.Y.).

PY - 1995/2/9

Y1 - 1995/2/9

N2 - We have studied the neutral glycolipid composition of spontaneous hepatomas in LEC female rats. Neutral lipid fractions were isolated and purified by column chromatographies on DEAE-Toyopearl 650(M) and Iatrobeads. The neutral glycolipid fraction contained 3.2 to 4.4 μg lipid-bound glucose (Glc) per mg protein, and consisted of isogloboside (iso-Gb4, 50.8% of total neutral glycolipids) and IV3Gal, IV2Fuc, GgOse4Cer (asialo-BGM1, 13.5%) as the major neutral glycolipids and Gb3 and iso-Gb3 (9.2%), GlcCer (7.2%), LacCer (6.1%) as the other species. The structure of iso-Gb4 was elucidated by gas-liquid chromatography (GLC), permethylation study, liquid secondary ion (LSI) mass spectrometry, and nuclear Overhauser enhancement spectroscopy (NOESY) and that for asialo-BGM1 by GLC, LSI mass spectrometry, and high-performance thin-layer chromatography (HPTLC)-overlay method using anti-asialo-BGM1 antibody. Isogloboside and asialo-BGM1 which are found in negligible amounts in normal liver tissues may represent excellent markers for studying tumor metastasis and cellular adhesion.

AB - We have studied the neutral glycolipid composition of spontaneous hepatomas in LEC female rats. Neutral lipid fractions were isolated and purified by column chromatographies on DEAE-Toyopearl 650(M) and Iatrobeads. The neutral glycolipid fraction contained 3.2 to 4.4 μg lipid-bound glucose (Glc) per mg protein, and consisted of isogloboside (iso-Gb4, 50.8% of total neutral glycolipids) and IV3Gal, IV2Fuc, GgOse4Cer (asialo-BGM1, 13.5%) as the major neutral glycolipids and Gb3 and iso-Gb3 (9.2%), GlcCer (7.2%), LacCer (6.1%) as the other species. The structure of iso-Gb4 was elucidated by gas-liquid chromatography (GLC), permethylation study, liquid secondary ion (LSI) mass spectrometry, and nuclear Overhauser enhancement spectroscopy (NOESY) and that for asialo-BGM1 by GLC, LSI mass spectrometry, and high-performance thin-layer chromatography (HPTLC)-overlay method using anti-asialo-BGM1 antibody. Isogloboside and asialo-BGM1 which are found in negligible amounts in normal liver tissues may represent excellent markers for studying tumor metastasis and cellular adhesion.

KW - Blood group B-active glycolipid

KW - Hepatoma

KW - Isogloboside

KW - LEC rat

KW - Neutral glycolipid

UR - https://www.scopus.com/pages/publications/0028859808

UR - https://www.scopus.com/pages/publications/0028859808#tab=citedBy

U2 - 10.1016/0005-2760(94)00186-3

DO - 10.1016/0005-2760(94)00186-3

M3 - Article

C2 - 7532009

AN - SCOPUS:0028859808

SN - 0005-2760

VL - 1254

SP - 257

EP - 266

JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism

JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism

IS - 3

ER -

Accumulation of isogloboside and ganglio-N-tetraosyl ceramide having blood group B determinant in the hepatomas of female LEC rats

Abstract

Keywords

ASJC Scopus subject areas

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