Adhesion development and the expression of endothelial nitric oxide synthase

David M. Svinarich; Fadi M. Zaher; Lena Holmdahl; Nasser Chegini; Bernard Gonik; Michael P. Diamond

doi:10.1155/S1064744901000199

Adhesion development and the expression of endothelial nitric oxide synthase

David M. Svinarich, Fadi M. Zaher, Lena Holmdahl, Nasser Chegini, Bernard Gonik, Michael P. Diamond

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Objective: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions. Methods: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and β-actin mRNA levels. Results: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were ≤ 3.12 × 10^-4, ≤ 3.12 × 10^-4, 6.24 × 10^-4 and 2.5 × 10^-3 attomoles/μl, respectively. β-actin mRNA levels for all tissues were between 1.25 × 10^-1 and 6.25 × 10^-2 attomoles/μl. Conclusion: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.

Original language	English (US)
Pages (from-to)	113-116
Number of pages	4
Journal	Infectious Diseases in Obstetrics and Gynecology
Volume	9
Issue number	2
DOIs	https://doi.org/10.1155/S1064744901000199
State	Published - 2001
Externally published	Yes

Keywords

Adhesions
QRT-PCR
Transcriptional Regulation
eNos

ASJC Scopus subject areas

Dermatology
Obstetrics and Gynecology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1155/S1064744901000199

Cite this

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title = "Adhesion development and the expression of endothelial nitric oxide synthase",

abstract = "Objective: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions. Methods: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and β-actin mRNA levels. Results: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were ≤ 3.12 × 10-4, ≤ 3.12 × 10-4, 6.24 × 10-4 and 2.5 × 10-3 attomoles/μl, respectively. β-actin mRNA levels for all tissues were between 1.25 × 10-1 and 6.25 × 10-2 attomoles/μl. Conclusion: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.",

keywords = "Adhesions, QRT-PCR, Transcriptional Regulation, eNos",

author = "Svinarich, {David M.} and Zaher, {Fadi M.} and Lena Holmdahl and Nasser Chegini and Bernard Gonik and Diamond, {Michael P.}",

year = "2001",

doi = "10.1155/S1064744901000199",

language = "English (US)",

volume = "9",

pages = "113--116",

journal = "Infectious Diseases in Obstetrics and Gynecology",

issn = "1064-7449",

publisher = "Hindawi Publishing Corporation",

number = "2",

}

TY - JOUR

T1 - Adhesion development and the expression of endothelial nitric oxide synthase

AU - Svinarich, David M.

AU - Zaher, Fadi M.

AU - Holmdahl, Lena

AU - Chegini, Nasser

AU - Gonik, Bernard

AU - Diamond, Michael P.

PY - 2001

Y1 - 2001

N2 - Objective: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions. Methods: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and β-actin mRNA levels. Results: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were ≤ 3.12 × 10-4, ≤ 3.12 × 10-4, 6.24 × 10-4 and 2.5 × 10-3 attomoles/μl, respectively. β-actin mRNA levels for all tissues were between 1.25 × 10-1 and 6.25 × 10-2 attomoles/μl. Conclusion: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.

AB - Objective: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions. Methods: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and β-actin mRNA levels. Results: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were ≤ 3.12 × 10-4, ≤ 3.12 × 10-4, 6.24 × 10-4 and 2.5 × 10-3 attomoles/μl, respectively. β-actin mRNA levels for all tissues were between 1.25 × 10-1 and 6.25 × 10-2 attomoles/μl. Conclusion: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.

KW - Adhesions

KW - QRT-PCR

KW - Transcriptional Regulation

KW - eNos

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JO - Infectious Diseases in Obstetrics and Gynecology

JF - Infectious Diseases in Obstetrics and Gynecology

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ER -

Adhesion development and the expression of endothelial nitric oxide synthase

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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