Abstract
The calcium sequestration characteristics of microsomal and mitochondrial fractions prepared from aortae of spontaneously hypertensive (SHR), Kyoto Wistar normotensive (NWR), and normotensive Sprague-Dawley (NSDR) rats were studied. Calcium uptake by the microsomal vesicles of SHR was significantly lower as compared to NWR and NSDR. However, mitochondria isolated from SHR aortae did not differ from NWR in their calcium uptake characteristics. Microsomal vesicles of NSDR aortae accumulated consistently more calcium than NWR. These findings illustrate the significance of relevant controls for SHR. Calcium-dependent ATPase activity in the microsomal fraction was significantly increased in SHR as compared to NWR. This may indicate a compensatory phenomenon on the part of the smooth muscle cell to increase Ca2+ uptake by the microsomal vesicles. These results suggest that a decreased ability to sequester calcium by the microsomal fraction of vascular smooth muscle of SHR may result in altered calcium distribution in the muscle cell.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 651-661 |
| Number of pages | 11 |
| Journal | Journal of molecular and cellular cardiology |
| Volume | 8 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 1976 |
Keywords
- Aortic smooth muscle
- Calcium sequestration
- Microsomal vesicles
- Mitochondria
- Spontaneously hypertensive rats
- Subcellular calcium distribution
ASJC Scopus subject areas
- Molecular Biology
- Cardiology and Cardiovascular Medicine