TY - JOUR
T1 - An evaluation of microbiologic contamination on a phosphor plate system
T2 - is weekly gas sterilization enough?
AU - Kalathingal, Sajitha
AU - Youngpeter, Alison
AU - Minton, Jason
AU - Shrout, Michael
AU - Dickinson, Douglas
AU - Plummer, Kevin
AU - Looney, Stephen
PY - 2010/3
Y1 - 2010/3
N2 - Objective: This study was performed to determine: 1) the rate and source of microbiologic contamination of photostimulable phosphor plates (PSP) in a predoctoral clinic; and 2) whether a combination of weekly gas sterilization and barrier protocols can prevent contamination of PSP plates. Methods: Fifty plates in clinical use and 25 gas-sterilized control plates were selected for examination. The PSP plates were pressed onto blood agar medium and incubated at 37°C. The number, size, distribution, and variety of resulting colonies were noted. To test whether these bacteria could have come from oral sources, 17 colonies were selected for culture on Mitis-Salivarius (M-S) agar. Those colonies that grew on the M-S agar were Gram stained. Results: Twenty-eight test plates (56%) exhibited growth of bacterial colonies on blood agar. Seventeen of those bacterial colonies were selected for growth on M-S agar, and 13 (76.47%) showed growth, 69% of them gram positive. Conclusion: Our results indicate reinforcing standard precautions of infection control for intraoral radiography, and that gas sterilization of plates after each day's clinical use is a potential solution.
AB - Objective: This study was performed to determine: 1) the rate and source of microbiologic contamination of photostimulable phosphor plates (PSP) in a predoctoral clinic; and 2) whether a combination of weekly gas sterilization and barrier protocols can prevent contamination of PSP plates. Methods: Fifty plates in clinical use and 25 gas-sterilized control plates were selected for examination. The PSP plates were pressed onto blood agar medium and incubated at 37°C. The number, size, distribution, and variety of resulting colonies were noted. To test whether these bacteria could have come from oral sources, 17 colonies were selected for culture on Mitis-Salivarius (M-S) agar. Those colonies that grew on the M-S agar were Gram stained. Results: Twenty-eight test plates (56%) exhibited growth of bacterial colonies on blood agar. Seventeen of those bacterial colonies were selected for growth on M-S agar, and 13 (76.47%) showed growth, 69% of them gram positive. Conclusion: Our results indicate reinforcing standard precautions of infection control for intraoral radiography, and that gas sterilization of plates after each day's clinical use is a potential solution.
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U2 - 10.1016/j.tripleo.2009.09.035
DO - 10.1016/j.tripleo.2009.09.035
M3 - Article
C2 - 20060337
AN - SCOPUS:77249109250
SN - 1079-2104
VL - 109
SP - 457
EP - 462
JO - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
JF - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
IS - 3
ER -