An in vitro evaluation of the cytotoxicity of various endodontic irrigants on human gingival fibroblasts

Brian D. Barnhart; Augustine Chuang; Jurandir J.Dalle Lucca; Steven Roberts; Frederick Liewehr; Anthony P. Joyce

doi:10.1097/01.don.0000153840.94227.87

An in vitro evaluation of the cytotoxicity of various endodontic irrigants on human gingival fibroblasts

Brian D. Barnhart, Augustine Chuang, Jurandir J.Dalle Lucca, Steven Roberts, Frederick Liewehr, Anthony P. Joyce

Endodontics

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

The purpose of this study was to measure the cytotoxicity of six endodontic irrigants on cultured gingival fibroblasts using the CyQuant assay. Human gingival fibroblasts were grown in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum at 37°C and 5% CO₂. At confluence, cells were split, plated in 96-well plates and incubated for 24-h to allow attachment. The following irrigants were tested at various concentrations: Sodium hypochlorite (NaOCl); iodine potassium-iodide (IKI); Betadine scrub (BS); calcium hydroxide [Ca(OH)₂]; chlorine dioxide (SCD) and DMEM (positive control). Experimental groups were compared by the logarithmic difference between the clinical and LD₅₀ concentrations of a particular irrigant. The results showed that IKI and Ca(OH)₂ were significantly less cytotoxic than SCD, NaOCl, and BS. In conclusion, IKI and Ca(OH)₂ are well tolerated by human gingival fibroblasts.

Original language	English (US)
Pages (from-to)	613-615
Number of pages	3
Journal	Journal of endodontics
Volume	31
Issue number	8
DOIs	https://doi.org/10.1097/01.don.0000153840.94227.87
State	Published - Aug 2005

Keywords

Betadine scrub
Calcium hydroxide
Chlorine dioxide
Iodine potassium-iodide
Sodium hypochlorite

ASJC Scopus subject areas

Dentistry(all)

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10.1097/01.don.0000153840.94227.87

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title = "An in vitro evaluation of the cytotoxicity of various endodontic irrigants on human gingival fibroblasts",

abstract = "The purpose of this study was to measure the cytotoxicity of six endodontic irrigants on cultured gingival fibroblasts using the CyQuant assay. Human gingival fibroblasts were grown in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum at 37°C and 5% CO2. At confluence, cells were split, plated in 96-well plates and incubated for 24-h to allow attachment. The following irrigants were tested at various concentrations: Sodium hypochlorite (NaOCl); iodine potassium-iodide (IKI); Betadine scrub (BS); calcium hydroxide [Ca(OH)2]; chlorine dioxide (SCD) and DMEM (positive control). Experimental groups were compared by the logarithmic difference between the clinical and LD50 concentrations of a particular irrigant. The results showed that IKI and Ca(OH)2 were significantly less cytotoxic than SCD, NaOCl, and BS. In conclusion, IKI and Ca(OH)2 are well tolerated by human gingival fibroblasts.",

keywords = "Betadine scrub, Calcium hydroxide, Chlorine dioxide, Iodine potassium-iodide, Sodium hypochlorite",

author = "Barnhart, {Brian D.} and Augustine Chuang and Lucca, {Jurandir J.Dalle} and Steven Roberts and Frederick Liewehr and Joyce, {Anthony P.}",

year = "2005",

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AU - Barnhart, Brian D.

AU - Chuang, Augustine

AU - Lucca, Jurandir J.Dalle

AU - Roberts, Steven

AU - Liewehr, Frederick

AU - Joyce, Anthony P.

PY - 2005/8

Y1 - 2005/8

N2 - The purpose of this study was to measure the cytotoxicity of six endodontic irrigants on cultured gingival fibroblasts using the CyQuant assay. Human gingival fibroblasts were grown in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum at 37°C and 5% CO2. At confluence, cells were split, plated in 96-well plates and incubated for 24-h to allow attachment. The following irrigants were tested at various concentrations: Sodium hypochlorite (NaOCl); iodine potassium-iodide (IKI); Betadine scrub (BS); calcium hydroxide [Ca(OH)2]; chlorine dioxide (SCD) and DMEM (positive control). Experimental groups were compared by the logarithmic difference between the clinical and LD50 concentrations of a particular irrigant. The results showed that IKI and Ca(OH)2 were significantly less cytotoxic than SCD, NaOCl, and BS. In conclusion, IKI and Ca(OH)2 are well tolerated by human gingival fibroblasts.

AB - The purpose of this study was to measure the cytotoxicity of six endodontic irrigants on cultured gingival fibroblasts using the CyQuant assay. Human gingival fibroblasts were grown in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum at 37°C and 5% CO2. At confluence, cells were split, plated in 96-well plates and incubated for 24-h to allow attachment. The following irrigants were tested at various concentrations: Sodium hypochlorite (NaOCl); iodine potassium-iodide (IKI); Betadine scrub (BS); calcium hydroxide [Ca(OH)2]; chlorine dioxide (SCD) and DMEM (positive control). Experimental groups were compared by the logarithmic difference between the clinical and LD50 concentrations of a particular irrigant. The results showed that IKI and Ca(OH)2 were significantly less cytotoxic than SCD, NaOCl, and BS. In conclusion, IKI and Ca(OH)2 are well tolerated by human gingival fibroblasts.

KW - Betadine scrub

KW - Calcium hydroxide

KW - Chlorine dioxide

KW - Iodine potassium-iodide

KW - Sodium hypochlorite

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An in vitro evaluation of the cytotoxicity of various endodontic irrigants on human gingival fibroblasts

Abstract

Keywords

ASJC Scopus subject areas

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