TY - JOUR
T1 - An invertebrate defense molecule activates membrane conductance in mammalian cells by means of its lectin-like domain
AU - Bloc, Alain
AU - Lucas, Rudolf
AU - Van Dijck, Els
AU - Bilej, Martin
AU - Dunant, Yves
AU - De Baetselier, Patrick
AU - Beschin, Alain
N1 - Funding Information:
This work was supported by the NATO Collaborative Linkage Grant (LST.CLG974974), the Foundation for Scientific Research-Flanders (G.0169.01), the Grant Agency of the Czech Republic (310/99/1385 and 310/00/1372) and a grant from the Swiss National Foundation for Scientific Research (31.57135.99) and was performed in the frame of an Interuniversity Attraction Pole Program
PY - 2002
Y1 - 2002
N2 - The invertebrate defense molecule Coelomic Cytolytic Factor-1 (CCF-1) and the mammalian cytokine Tumor Necrosis Factor (TNF) share a similar lectin-like domain that, upon interaction with specific sugars, causes lysis of African trypanosomes. In contrast to TNF, CCF-1 does not require an acidification of a lysosomal compartment for this activity. Moreover, we could demonstrate using the whole cell patch clamp technique that both TNF and CCF-1 activate amiloride-sensitive channels in mammalian cells, in a TNF receptor-independent way, but, unlike TNF, CCF-1 does not require acidic conditions for this activity. These data confirm the functional analogies of an invertebrate defense molecule and a mammalian cytokine, based on a similar lectin-like interaction.
AB - The invertebrate defense molecule Coelomic Cytolytic Factor-1 (CCF-1) and the mammalian cytokine Tumor Necrosis Factor (TNF) share a similar lectin-like domain that, upon interaction with specific sugars, causes lysis of African trypanosomes. In contrast to TNF, CCF-1 does not require an acidification of a lysosomal compartment for this activity. Moreover, we could demonstrate using the whole cell patch clamp technique that both TNF and CCF-1 activate amiloride-sensitive channels in mammalian cells, in a TNF receptor-independent way, but, unlike TNF, CCF-1 does not require acidic conditions for this activity. These data confirm the functional analogies of an invertebrate defense molecule and a mammalian cytokine, based on a similar lectin-like interaction.
KW - Amiloride-sensitive conductance
KW - CCF-1
KW - Innate immunity
KW - Lectin
KW - TNF
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U2 - 10.1016/S0145-305X(01)00054-4
DO - 10.1016/S0145-305X(01)00054-4
M3 - Article
C2 - 11687261
AN - SCOPUS:0036027774
SN - 0145-305X
VL - 26
SP - 35
EP - 43
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 1
ER -