Analysis of aflatoxin B1 in human tissues with high-pressure liquid chromatography

Mohamed Y. Siraj; A. Wallace Hayes; Peter D. Unger; Gwendolyn R. Hogan; Nell J. Ryan; Betty B. Wray

doi:10.1016/0041-008X(81)90094-6

Analysis of aflatoxin B₁ in human tissues with high-pressure liquid chromatography

Mohamed Y. Siraj, A. Wallace Hayes, Peter D. Unger, Gwendolyn R. Hogan, Nell J. Ryan, Betty B. Wray

Allergy-Immunology and Rheumatology

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Fast, reproducible, and sensitive methods for the extraction and analysis of aflatoxin B₁ in human liver, blood, and urine by high-pressure liquid chromatography are described. Recovery of aflatoxin B₁ from spiked mouse liver homogenates was quantitative (>97%) over a range of 1.6-1600 ng/g. Better than 79% recovery of aflatoxin B₁ was obtained from human blood samples spiked with 1-50 ng/ml. Using these procedures, liver samples from patients with primary hepatocellular carcinoma and liver, blood, and urine from patients diagnosed with Reye's syndrome were analyzed for aflatoxin B₁. Aflatoxin B₁ was detected in 4 of the 6 liver samples from primary hepatocellular carcinoma patients. Aflatoxin B₁ also was detected in 11 of 14 liver samples, 4 of 6 blood samples, and 4 of 5 urine samples from patients with Reye's syndrome.

Original language	English (US)
Pages (from-to)	422-430
Number of pages	9
Journal	Toxicology and Applied Pharmacology
Volume	58
Issue number	3
DOIs	https://doi.org/10.1016/0041-008X(81)90094-6
State	Published - May 1981

ASJC Scopus subject areas

Toxicology
Pharmacology

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title = "Analysis of aflatoxin B1 in human tissues with high-pressure liquid chromatography",

abstract = "Fast, reproducible, and sensitive methods for the extraction and analysis of aflatoxin B1 in human liver, blood, and urine by high-pressure liquid chromatography are described. Recovery of aflatoxin B1 from spiked mouse liver homogenates was quantitative (>97%) over a range of 1.6-1600 ng/g. Better than 79% recovery of aflatoxin B1 was obtained from human blood samples spiked with 1-50 ng/ml. Using these procedures, liver samples from patients with primary hepatocellular carcinoma and liver, blood, and urine from patients diagnosed with Reye's syndrome were analyzed for aflatoxin B1. Aflatoxin B1 was detected in 4 of the 6 liver samples from primary hepatocellular carcinoma patients. Aflatoxin B1 also was detected in 11 of 14 liver samples, 4 of 6 blood samples, and 4 of 5 urine samples from patients with Reye's syndrome.",

author = "Siraj, {Mohamed Y.} and Hayes, {A. Wallace} and Unger, {Peter D.} and Hogan, {Gwendolyn R.} and Ryan, {Nell J.} and Wray, {Betty B.}",

note = "Funding Information: This investigation was supported by USPHS ES-01351 and ES-02191.",

year = "1981",

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AU - Siraj, Mohamed Y.

AU - Hayes, A. Wallace

AU - Unger, Peter D.

AU - Hogan, Gwendolyn R.

AU - Ryan, Nell J.

AU - Wray, Betty B.

N1 - Funding Information: This investigation was supported by USPHS ES-01351 and ES-02191.

PY - 1981/5

Y1 - 1981/5

N2 - Fast, reproducible, and sensitive methods for the extraction and analysis of aflatoxin B1 in human liver, blood, and urine by high-pressure liquid chromatography are described. Recovery of aflatoxin B1 from spiked mouse liver homogenates was quantitative (>97%) over a range of 1.6-1600 ng/g. Better than 79% recovery of aflatoxin B1 was obtained from human blood samples spiked with 1-50 ng/ml. Using these procedures, liver samples from patients with primary hepatocellular carcinoma and liver, blood, and urine from patients diagnosed with Reye's syndrome were analyzed for aflatoxin B1. Aflatoxin B1 was detected in 4 of the 6 liver samples from primary hepatocellular carcinoma patients. Aflatoxin B1 also was detected in 11 of 14 liver samples, 4 of 6 blood samples, and 4 of 5 urine samples from patients with Reye's syndrome.

AB - Fast, reproducible, and sensitive methods for the extraction and analysis of aflatoxin B1 in human liver, blood, and urine by high-pressure liquid chromatography are described. Recovery of aflatoxin B1 from spiked mouse liver homogenates was quantitative (>97%) over a range of 1.6-1600 ng/g. Better than 79% recovery of aflatoxin B1 was obtained from human blood samples spiked with 1-50 ng/ml. Using these procedures, liver samples from patients with primary hepatocellular carcinoma and liver, blood, and urine from patients diagnosed with Reye's syndrome were analyzed for aflatoxin B1. Aflatoxin B1 was detected in 4 of the 6 liver samples from primary hepatocellular carcinoma patients. Aflatoxin B1 also was detected in 11 of 14 liver samples, 4 of 6 blood samples, and 4 of 5 urine samples from patients with Reye's syndrome.

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ER -

Analysis of aflatoxin B₁ in human tissues with high-pressure liquid chromatography

Abstract

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