Analysis of rat calvaria defects implanted with a platelet-rich plasma preparation: Radiographic observations

Mary E. Pryor, Jie Yang, Giuseppe Polimeni, Ki Tae Koo, Michael J. Hartman, Howard Gross, Alexis Agelan, Joanne M. Manns, Ulf M E Wikesjö

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24 Scopus citations


Background: Platelet-rich plasma (PRP) harbors growth factors identified in bone. It has been suggested that these factors enhance osteogenesis. The objective of this study was to conduct a radiographic evaluation on local bone formation following surgical implantation of a PRP preparation using a critical-size rat calvaria defect model. Methods: Thirty 22-week-old male Sprague-Dawley rats were used. The PRP preparation was obtained from 10 ml of whole blood drawn from one age-matched donor rat. The preparation was processed by gradient density centrifugation and stored at -80°C until use. Using aseptic techniques, the PRP preparation soak-loaded onto an absorbable collagen sponge (ACS) carrier or ACS alone was surgically implanted into contralateral critical-size 6 mm rat calvaria osteotomies in 18 animals. Twelve animals received ACS alone versus sham surgery in contralateral defects. Animals were sacrificed at 4 and 8 weeks when biopsies were collected and radiographs were obtained using a standardized protocol. Three masked examiners independently evaluated the radiographic images of the defect sites. Examiner reproducibility was examined by repeat evaluation of all defect sites (r = 0.6; P<0.0001). Results: The animals were maintained without adverse events. Defect sites in two animals receiving ACS versus sham surgery (4-week healing interval) were not evaluated due to specimen damage. Seventy-five percent of the sites (PRP/ACS or ACS) exhibited partial closure at 4 weeks; one site (ACS) exhibited full closure without significant differences between protocols (P = 0.1797). Fifty percent of the sites receiving PRP/ACS exhibited full closure and 20% partial closure at 8 weeks versus 20% and 80%, respectively, for the ACS control (P= 0.7532). There were no noteworthy differences between sites receiving ACS versus sham surgery at 4 or 8 weeks. Conclusion: The results suggest that the PRP preparation does not have a significant effect on osteogenesis.

Original languageEnglish (US)
Pages (from-to)1287-1292
Number of pages6
JournalJournal of periodontology
Issue number8
StatePublished - Aug 2005


  • Animal studies
  • Bone and bones
  • Growth factors
  • Osteogenesis
  • Plasma, platelet-rich
  • Tissue engineering

ASJC Scopus subject areas

  • Periodontics


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