TY - JOUR
T1 - AngII induces transient phospholipase D activity in the H295R glomerulosa cell model
AU - Zheng, Xiangjian
AU - Bollag, Wendy B.
N1 - Funding Information:
We thank Dr Bill Rainey (University of Texas Southwestern Medical Center, Dallas, TX) for his generous gift of H295R cells and Mrs Patricia Kent for her assistance with their culture. This work was supported by American Heart Association/Southeastern affiliate award #0051579B and American Heart Association National Grant-in-Aid award #0350166N (to WBB), and was completed in partial fulfillment of the requirements for a Doctor of Philosophy (XZ).
PY - 2003/8/29
Y1 - 2003/8/29
N2 - In this report we demonstrate that in human adrenocortical carcinoma NCI H295R cells, a model for adrenal glomerulosa cells, PLD was activated both by AngII and protein kinase C (PKC)-activating phorbol 12-myristate 13-acetate (PMA). However, while PMA triggered sustained PLD activation, AngII induced transient PLD activation, in contrast to results in bovine glomerulosa cells in primary culture. Despite the transient effect of AngII on PLD activity, PLD-derived lipid signals were required for maximal AngII-elicited aldosterone secretion. AngII-induced PLD activation was inhibited by PKC inhibitors, but not by tyrosine kinase or calcium/calmodulin-dependent kinase inhibitors or a calmodulin antagonist. Both AngII- and PMA-stimulated PLD activity was enhanced by phosphoinositide 3-kinase (PI3K) inhibitors. Akt, a downstream protein kinase activated by the products of PI3K, was constitutively active in H295R cells, and this activity was blocked by PI3K inhibitors. These results suggested that in H295R adrenocortical carcinoma cells, AngII-induced PLD activation was promoted by PKC and inhibited by the constitutively active PI3K pathway.
AB - In this report we demonstrate that in human adrenocortical carcinoma NCI H295R cells, a model for adrenal glomerulosa cells, PLD was activated both by AngII and protein kinase C (PKC)-activating phorbol 12-myristate 13-acetate (PMA). However, while PMA triggered sustained PLD activation, AngII induced transient PLD activation, in contrast to results in bovine glomerulosa cells in primary culture. Despite the transient effect of AngII on PLD activity, PLD-derived lipid signals were required for maximal AngII-elicited aldosterone secretion. AngII-induced PLD activation was inhibited by PKC inhibitors, but not by tyrosine kinase or calcium/calmodulin-dependent kinase inhibitors or a calmodulin antagonist. Both AngII- and PMA-stimulated PLD activity was enhanced by phosphoinositide 3-kinase (PI3K) inhibitors. Akt, a downstream protein kinase activated by the products of PI3K, was constitutively active in H295R cells, and this activity was blocked by PI3K inhibitors. These results suggested that in H295R adrenocortical carcinoma cells, AngII-induced PLD activation was promoted by PKC and inhibited by the constitutively active PI3K pathway.
KW - Adrenocortical carcinoma
KW - Aldosterone
KW - NCI H295R cells
KW - Phosphatidic acid
KW - Phosphoinositide 3-kinase
KW - Protein kinase C
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U2 - 10.1016/S0303-7207(03)00211-9
DO - 10.1016/S0303-7207(03)00211-9
M3 - Article
C2 - 12943994
AN - SCOPUS:0041430880
SN - 0303-7207
VL - 206
SP - 113
EP - 122
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-2
ER -