Abstract
Objective: To determine whether docosahexaenoic acid (DHA) reduces adhesion marker mRNA levels in normal peritoneal and adhesion fibroblasts. Design: Prospective experimental study. Setting: University Medical Center. Patient(s): Three patients undergoing laparotomy with excision of adhesions and normal peritoneum. Intervention(s): DHA treatment (100 μM) of cell cultures for 24 hours. Main Outcome Measure(s): Real-time reverse transcriptase polymerase chain reaction (RT-PCR) quantification of relative changes (mRNA copies/μg mRNA) in mRNA levels of type I collagen, vascular endothelial growth factor (VEGF), and transforming growth factor-β1 (TGF-β1). Result(s): The DHA treatment significantly reduced type I collagen and VEGF, but not TGF-β1 mRNA levels in normal peritoneal fibroblasts compared to normal controls. The DHA treatment of adhesion fibroblasts reduced type I collagen mRNAs to those of normal peritoneal fibroblasts, decreasing mRNAs by 35% compared to untreated adhesion fibroblasts. The VEGF mRNA levels were 50% lower in DHA-treated adhesion fibroblasts versus untreated adhesion fibroblasts. Docasahexaenoic acid reduced TGF- β1 mRNA to normal levels in treated adhesion fibroblasts compared to untreated normal peritoneal fibroblasts. Conclusion(s): Docasahexaenoic acid substantially reduces levels of adhesion-related markers in normal peritoneal and adhesion fibroblasts. This study provides the molecular basis for an easily administered and potentially, highly efficacious, antiadhesion adjuvant.
Original language | English (US) |
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Pages (from-to) | 1657-1662 |
Number of pages | 6 |
Journal | Fertility and sterility |
Volume | 88 |
Issue number | 6 |
DOIs | |
State | Published - Dec 2007 |
Externally published | Yes |
Keywords
- DHA
- Docosahexaenoic acid
- adhesion barriers
- adhesions
- inflammation
- omega 3
- peritoneum
- transforming growth factor-β1
- type I collagen
- vascular endothelial growth factor
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology