TY - JOUR
T1 - Antibacterial effects and physical properties of glass-ionomer cements containing chlorhexidine for the ART approach
AU - Takahashi, Yusuke
AU - Imazato, Satoshi
AU - Kaneshiro, Andrea V.
AU - Ebisu, Shigeyuki
AU - Frencken, Jo E.
AU - Tay, Franklin R.
N1 - Funding Information:
This study was supported by a Grant-in-aid for Scientific Research (15209066, 16390545, 17791355) from the Japan Society for the Promotion of Science, and the 21st Century COE entitled ‘Origination of Frontier BioDentistry’ at Osaka University Graduate School of Dentistry supported by the Ministry of Education, Culture, Sports, Science and Technology. A research grant from Daiwa Securities Health Foundation also supported this investigation.
PY - 2006/7
Y1 - 2006/7
N2 - Objectives: Since atraumatic restorative treatment (ART) involves removal of carious lesions with manual instruments, improvement of filling materials to guarantee greater success should be considered. This study aimed to evaluate antibacterial, physical, and bonding properties of glass-ionomer cements (GIC) containing chlorhexidine (CHX), and to determine optimal concentrations for incorporation of agents to obtain antibacterial GICs for use with the ART approach. Methods: CHX diacetate combined with CHX dihydrochloride was added to control GIC powder to obtain concentration ratios of 1/0, 2/0, 3/0, 1/1, or 2/2% w/w. Antibacterial activity of each cement against Streptococcus mutans, Lactobacillus casei or Actinomyces naeslundii was examined using agar-diffusion methods, and release of CHX was analyzed by HPLC. Compressive strength, bond strength to dentin, and setting time were measured, and compared with those of control samples. Results: All experimental GICs exhibited inhibition of three bacteria, but sizes of inhibition zones and concentrations of CHX released were not dependent upon CHX content. Incorporation of CHX diacetate at 2% or greater, significantly decreased compressive strength, and bond strength to dentin was adversely affected by addition of CHX diacetate at 2% or more (p<0.05, ANOVA, Fisher's PLSD test), although setting time was extended a little by addition of any concentrations of CHX. Significance: The present results demonstrate that experimental GICs containing CHX are effective in inhibiting bacteria associated with caries, and incorporation of 1% CHX diacetate is optimal to give appropriate physical and bonding properties.
AB - Objectives: Since atraumatic restorative treatment (ART) involves removal of carious lesions with manual instruments, improvement of filling materials to guarantee greater success should be considered. This study aimed to evaluate antibacterial, physical, and bonding properties of glass-ionomer cements (GIC) containing chlorhexidine (CHX), and to determine optimal concentrations for incorporation of agents to obtain antibacterial GICs for use with the ART approach. Methods: CHX diacetate combined with CHX dihydrochloride was added to control GIC powder to obtain concentration ratios of 1/0, 2/0, 3/0, 1/1, or 2/2% w/w. Antibacterial activity of each cement against Streptococcus mutans, Lactobacillus casei or Actinomyces naeslundii was examined using agar-diffusion methods, and release of CHX was analyzed by HPLC. Compressive strength, bond strength to dentin, and setting time were measured, and compared with those of control samples. Results: All experimental GICs exhibited inhibition of three bacteria, but sizes of inhibition zones and concentrations of CHX released were not dependent upon CHX content. Incorporation of CHX diacetate at 2% or greater, significantly decreased compressive strength, and bond strength to dentin was adversely affected by addition of CHX diacetate at 2% or more (p<0.05, ANOVA, Fisher's PLSD test), although setting time was extended a little by addition of any concentrations of CHX. Significance: The present results demonstrate that experimental GICs containing CHX are effective in inhibiting bacteria associated with caries, and incorporation of 1% CHX diacetate is optimal to give appropriate physical and bonding properties.
KW - Antibacterial effects
KW - Atraumatic restorative treatment
KW - Bond strength
KW - Chlorhexidine
KW - Compressive strength
KW - Glass-ionomer cement
KW - Setting time
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U2 - 10.1016/j.dental.2005.08.003
DO - 10.1016/j.dental.2005.08.003
M3 - Article
C2 - 16226806
AN - SCOPUS:33744908018
SN - 0109-5641
VL - 22
SP - 647
EP - 652
JO - Dental Materials
JF - Dental Materials
IS - 7
ER -