Antibacterial mesh: A novel technique involving naturally occurring cellular proteins

Yuliya Yurko, Kathleen McDeavitt, Rohan Satish Kumar, Terri Martin, Ajita Prabhu, Amy E. Lincourt, Alexey Vertegel, B. Todd Heniford

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Background. Naturally occurring antimicrobial peptides are possibly the "next frontier" in infection prevention. Binding them to mesh could reduce the rate of mesh infections. This study identifies an antimicrobial agent capable of significant antibacterial activity when bound to mesh. Methods. Lysozyme, human beta defensin (HBD-3), human cathelicidin (LL-37), and lysostaphin were adsorbed to polypropylene mesh at various concentrations. Treated meshes were placed in a suspension of 1 × 106Staphylococcus aureus. Antibacterial action was monitored by turbidimetric assay, fluorescent imaging, and a colony counting method. Results. A very high rate of lysis of S aureus cells was observed in the lysostaphin-treated group as measured by optical density; none survived as seen on colony count assays. Optical density for mesh coated with lysozyme, HBD-3, and LL-37 did not differ from untreated controls, with 100% survival rates by colony counts. Conclusion. Lysostaphin had superior antibacterial activity following adsorption to mesh.

Original languageEnglish (US)
Pages (from-to)20-26
Number of pages7
JournalSurgical Innovation
Issue number1
StatePublished - Mar 2012


  • Staphylococcus aureus
  • adsorption
  • antimicrobial protein
  • lysostaphin
  • polypropylene mesh

ASJC Scopus subject areas

  • Surgery


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