Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells

L. H.C. Makala; Y. Nishikawa; T. Kamada; X. Xuan; H. Nagasawa

doi:10.1159/000048763

Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells

L. H.C. Makala, Y. Nishikawa, T. Kamada, X. Xuan, H. Nagasawa

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Murine peritoneal cavity macrophage derived dendritic cells (PEC-DC) generated using early growth factors, interleukin 4 and granulocyte-macrophage colony-stimulating factor followed by maturation in interferon-gamma plus either, Toxoplasma lysate antigen (TLA) or lipopolysaccharide, bind TLA by a nonspecific mechanism and continue to express major histocompatibility complex class II antigens after 24 h of culture in vitro. Moreover, the proliferation of CD3+ spleen T cells from mice immunized with Toxoplasma gondii homogenate, induced by PEC-DC-mediated antigen presentation was statistically significant and of consistent amplitude. This accessory function of PEC-DC is antigen specific.

Original language	English (US)
Pages (from-to)	104-112
Number of pages	9
Journal	Pathobiology
Volume	69
Issue number	2
DOIs	https://doi.org/10.1159/000048763
State	Published - Dec 1 2001
Externally published	Yes

Keywords

Antigen presentation
Mouse
Peritoneal macrophage-derived dendritic cell
Toxoplasma lysate antigen

ASJC Scopus subject areas

Pathology and Forensic Medicine
Molecular Biology
Cell Biology

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abstract = "Murine peritoneal cavity macrophage derived dendritic cells (PEC-DC) generated using early growth factors, interleukin 4 and granulocyte-macrophage colony-stimulating factor followed by maturation in interferon-gamma plus either, Toxoplasma lysate antigen (TLA) or lipopolysaccharide, bind TLA by a nonspecific mechanism and continue to express major histocompatibility complex class II antigens after 24 h of culture in vitro. Moreover, the proliferation of CD3+ spleen T cells from mice immunized with Toxoplasma gondii homogenate, induced by PEC-DC-mediated antigen presentation was statistically significant and of consistent amplitude. This accessory function of PEC-DC is antigen specific.",

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T1 - Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells

AU - Makala, L. H.C.

AU - Nishikawa, Y.

AU - Kamada, T.

AU - Xuan, X.

AU - Nagasawa, H.

PY - 2001/12/1

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AB - Murine peritoneal cavity macrophage derived dendritic cells (PEC-DC) generated using early growth factors, interleukin 4 and granulocyte-macrophage colony-stimulating factor followed by maturation in interferon-gamma plus either, Toxoplasma lysate antigen (TLA) or lipopolysaccharide, bind TLA by a nonspecific mechanism and continue to express major histocompatibility complex class II antigens after 24 h of culture in vitro. Moreover, the proliferation of CD3+ spleen T cells from mice immunized with Toxoplasma gondii homogenate, induced by PEC-DC-mediated antigen presentation was statistically significant and of consistent amplitude. This accessory function of PEC-DC is antigen specific.

KW - Antigen presentation

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KW - Toxoplasma lysate antigen

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Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells

Abstract

Keywords

ASJC Scopus subject areas

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