TY - JOUR
T1 - Aplysia mollusk-derived growth factor is a mitogen with adenosine deaminase activity and is expressed in the developing central nervous system
AU - Akalal, David Benjamin G.
AU - Bottenstein, Jane E.
AU - Lee, Seung Hee
AU - Han, Jin Hee
AU - Chang, Deog Jin
AU - Kaang, Bong Kiun
AU - Nagle, Gregg T.
N1 - Funding Information:
Supported by NSF IBN-9985778 (G.T.N.), National Research Laboratory Grant M1-0101-00-0140 (B.-K.K.), and Korea Science and Engineering Foundation Grant 1999-1-213-002-5 (B.-K.K.). We thank E. Sasser for assistance in MDGF purification and enzyme assays, and the UTMB Protein Chemistry Lab and Recombinant DNA Sequencing Core Facility for technical assistance.
PY - 2003/10/7
Y1 - 2003/10/7
N2 - Mollusk-derived growth factor (MDGF), the first growth factor to be characterized in Aplysia, was purified and characterized and has both adenosine deaminase activity and stimulates cell proliferation in vitro. MDGF is structurally related to a new subfamily of adenosine deaminase-related growth factors that require enzymatic activity to stimulate cell proliferation, a unique property of known growth factors. We examined the expression of MDGF protein in the CNS since MDGF mRNA increased in the developing CNS, and recent data suggest that inosine is involved in neuronal reorganization and restoration of essential circuitry after CNS injury. MDGF levels transiently increased during embryonic and post-metamorphic development and in the developing CNS, but was undetectable in adult CNS. No effects on morphology or neurite extension of adult Aplysia neurons were observed.
AB - Mollusk-derived growth factor (MDGF), the first growth factor to be characterized in Aplysia, was purified and characterized and has both adenosine deaminase activity and stimulates cell proliferation in vitro. MDGF is structurally related to a new subfamily of adenosine deaminase-related growth factors that require enzymatic activity to stimulate cell proliferation, a unique property of known growth factors. We examined the expression of MDGF protein in the CNS since MDGF mRNA increased in the developing CNS, and recent data suggest that inosine is involved in neuronal reorganization and restoration of essential circuitry after CNS injury. MDGF levels transiently increased during embryonic and post-metamorphic development and in the developing CNS, but was undetectable in adult CNS. No effects on morphology or neurite extension of adult Aplysia neurons were observed.
KW - ADA
KW - Adenosine deaminase-related growth factor
KW - Aplysia
KW - CECR1
KW - Developing central nervous system
KW - Insect-derived growth factor
KW - MDGF
KW - Neurotransmitters, modulators, transporters, and receptors
KW - Transmitters in invertebrates
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UR - http://www.scopus.com/inward/citedby.url?scp=1642361602&partnerID=8YFLogxK
U2 - 10.1016/S0169-328X(03)00287-0
DO - 10.1016/S0169-328X(03)00287-0
M3 - Article
C2 - 14559158
AN - SCOPUS:1642361602
SN - 0169-328X
VL - 117
SP - 228
EP - 236
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 2
ER -