Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection

Mustafa Culha; David L. Stokes; Guy D. Griffin; Tuan Vo-Dinh

doi:10.1016/j.bios.2003.09.006

Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection

Mustafa Culha, David L. Stokes, Guy D. Griffin, Tuan Vo-Dinh

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

We report for the first time the application of a biochip using the molecular beacon (MB) detection scheme. The usability of this biochip novel detection system for the analysis of the breast cancer gene BRCA1 is demonstrated using molecular beacon probes. The MB is designed for the BRCA1 gene and a miniature biochip system is used for detection. The performance of the biochip-MB detection system is evaluated. The optimum conditions for the MB system for highest fluorescence detection sensitivity are investigated for the detection system. The detection of BRCA1 gene is successfully demonstrated in solution and the limit of detection (LOD) is estimated as 70nM.

Original language	English (US)
Pages (from-to)	1007-1012
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	19
Issue number	9
DOIs	https://doi.org/10.1016/j.bios.2003.09.006
State	Published - Apr 15 2004
Externally published	Yes

Keywords

BRCA1
Biochip
Detection
Fluorescence
Molecular beacon

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bios.2003.09.006

Cite this

@article{aa23b76bb7af4692b73f33111fa62337,

title = "Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection",

abstract = "We report for the first time the application of a biochip using the molecular beacon (MB) detection scheme. The usability of this biochip novel detection system for the analysis of the breast cancer gene BRCA1 is demonstrated using molecular beacon probes. The MB is designed for the BRCA1 gene and a miniature biochip system is used for detection. The performance of the biochip-MB detection system is evaluated. The optimum conditions for the MB system for highest fluorescence detection sensitivity are investigated for the detection system. The detection of BRCA1 gene is successfully demonstrated in solution and the limit of detection (LOD) is estimated as 70nM.",

keywords = "BRCA1, Biochip, Detection, Fluorescence, Molecular beacon",

author = "Mustafa Culha and Stokes, {David L.} and Griffin, {Guy D.} and Tuan Vo-Dinh",

note = "Funding Information: This work was sponsored by the US Department of Energy (DOE), Office of Biological and Environmental Research, and the DOE, Chemical and Biological Nonproliferation Program and under contract DE-AC05-00OR22725 with UT-Battelle, LLC. Mustafa Culha and David Stokes are supported by an appointment to Oak Ridge National Laboratory Postdoctoral Research Associates Program, administered jointly by the Oak Ridge National Laboratory and Oak Ridge Institute for Science and Education.",

year = "2004",

month = apr,

day = "15",

doi = "10.1016/j.bios.2003.09.006",

language = "English (US)",

volume = "19",

pages = "1007--1012",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Limited",

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TY - JOUR

T1 - Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection

AU - Culha, Mustafa

AU - Stokes, David L.

AU - Griffin, Guy D.

AU - Vo-Dinh, Tuan

N1 - Funding Information: This work was sponsored by the US Department of Energy (DOE), Office of Biological and Environmental Research, and the DOE, Chemical and Biological Nonproliferation Program and under contract DE-AC05-00OR22725 with UT-Battelle, LLC. Mustafa Culha and David Stokes are supported by an appointment to Oak Ridge National Laboratory Postdoctoral Research Associates Program, administered jointly by the Oak Ridge National Laboratory and Oak Ridge Institute for Science and Education.

PY - 2004/4/15

Y1 - 2004/4/15

N2 - We report for the first time the application of a biochip using the molecular beacon (MB) detection scheme. The usability of this biochip novel detection system for the analysis of the breast cancer gene BRCA1 is demonstrated using molecular beacon probes. The MB is designed for the BRCA1 gene and a miniature biochip system is used for detection. The performance of the biochip-MB detection system is evaluated. The optimum conditions for the MB system for highest fluorescence detection sensitivity are investigated for the detection system. The detection of BRCA1 gene is successfully demonstrated in solution and the limit of detection (LOD) is estimated as 70nM.

AB - We report for the first time the application of a biochip using the molecular beacon (MB) detection scheme. The usability of this biochip novel detection system for the analysis of the breast cancer gene BRCA1 is demonstrated using molecular beacon probes. The MB is designed for the BRCA1 gene and a miniature biochip system is used for detection. The performance of the biochip-MB detection system is evaluated. The optimum conditions for the MB system for highest fluorescence detection sensitivity are investigated for the detection system. The detection of BRCA1 gene is successfully demonstrated in solution and the limit of detection (LOD) is estimated as 70nM.

KW - BRCA1

KW - Biochip

KW - Detection

KW - Fluorescence

KW - Molecular beacon

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UR - http://www.scopus.com/inward/citedby.url?scp=1342285629&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2003.09.006

DO - 10.1016/j.bios.2003.09.006

M3 - Article

C2 - 15018955

AN - SCOPUS:1342285629

SN - 0956-5663

VL - 19

SP - 1007

EP - 1012

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 9

ER -

Application of a miniature biochip using the molecular beacon probe in breast cancer gene BRCA1 detection

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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