Aqueous extract of Trigonella foenum-graecum L. ameliorates additive urotoxicity of buthionine sulfoximine and cyclophosphamide in mice

K. Bhatia, M. Kaur, F. Atif, M. Ali, H. Rehman, S. Rahman, S. Raisuddin

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

Cyclophosphamide (CP) is a commonly used anti-cancer drug which causes toxicity by its reactive metabolites such as acrolein and phosphoramide mustard. In the present study modulation of toxicity caused by concomitant exposure to CP and l-buthionine-SR-sulfoximine (BSO) by fenugreek (Trigonella foenum-graecum L.) extract was evaluated by measuring lipid peroxidation (LPO) and anti-oxidants in urinary bladder in mice. Fenugreek, a common dietary and medicinal herb, showed protective effect not only on LPO but also on the enzymatic anti-oxidants. CP-treated animals exhibited a significant decrease in the activities of glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GP) and catalase (CAT) when compared to the controls. Level of reduced glutathione (GSH) was also reduced with an increase in LPO in CP-treated animals. BSO treatment depicted an additive toxic effect in CP-treated animals. Pre-treatment of herbal extract restored activities of all the enzymes and thus showed an overall protective effect on additive effect of CP and BSO. Restoration of GSH by extract treatment may play an important role in reversing CP-induced apoptosis and free radical-mediated LPO in urinary bladder. Fenugreek, known for its hypoglycemic, anti-inflammatory and immunomodulatory activity, may be a promising protective medicinal herb for consideration in complementary therapy in cancer patients under chemotherapeutic interventions.

Original languageEnglish (US)
Pages (from-to)1744-1750
Number of pages7
JournalFood and Chemical Toxicology
Volume44
Issue number10
DOIs
StatePublished - Oct 2006
Externally publishedYes

Keywords

  • BSO
  • Cyclophosphamide
  • Fenugreek extract
  • Protection
  • Urinary bladder anti-oxidants

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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