Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene

Shelly R. Gunn; Aswani R. Bolla; Lynn L. Barron; Mercedes E. Gorre; Mansoor S. Mohammed; David W. Bahler; Clemens H.M. Mellink; Marinus H.J. van Oers; Michael J. Keating; Alessandra Ferrajoli; Kevin R. Coombes; Lynne V. Abruzzo; Ryan S. Robetorye

doi:10.1016/j.leukres.2008.10.010

Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene

Shelly R. Gunn
, Aswani R. Bolla
, Lynn L. Barron
, Mercedes E. Gorre
, Mansoor S. Mohammed
, David W. Bahler
, Clemens H.M. Mellink
, Marinus H.J. van Oers
, Michael J. Keating
, Alessandra Ferrajoli
, Kevin R. Coombes
, Lynne V. Abruzzo
, Ryan S. Robetorye

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to ∼1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.

Original language	English (US)
Pages (from-to)	1276-1281
Number of pages	6
Journal	Leukemia Research
Volume	33
Issue number	9
DOIs	https://doi.org/10.1016/j.leukres.2008.10.010
State	Published - Sep 2009
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Array CGH
Chromosome 22q11
Chronic lymphocytic leukemia
PRAME gene

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/j.leukres.2008.10.010

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Gunn, S. R., Bolla, A. R., Barron, L. L., Gorre, M. E., Mohammed, M. S., Bahler, D. W., Mellink, C. H. M., van Oers, M. H. J., Keating, M. J., Ferrajoli, A., Coombes, K. R., Abruzzo, L. V., & Robetorye, R. S. (2009). Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene. Leukemia Research, 33(9), 1276-1281. https://doi.org/10.1016/j.leukres.2008.10.010

Gunn, SR, Bolla, AR, Barron, LL, Gorre, ME, Mohammed, MS, Bahler, DW, Mellink, CHM, van Oers, MHJ, Keating, MJ, Ferrajoli, A, Coombes, KR, Abruzzo, LV & Robetorye, RS 2009, 'Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene', Leukemia Research, vol. 33, no. 9, pp. 1276-1281. https://doi.org/10.1016/j.leukres.2008.10.010

@article{0d3d2c42cb8f4b2c927c22a9bf8b158f,

title = "Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene",

abstract = "We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15\%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to ∼1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.",

keywords = "Array CGH, Chromosome 22q11, Chronic lymphocytic leukemia, PRAME gene",

author = "Gunn, \{Shelly R.\} and Bolla, \{Aswani R.\} and Barron, \{Lynn L.\} and Gorre, \{Mercedes E.\} and Mohammed, \{Mansoor S.\} and Bahler, \{David W.\} and Mellink, \{Clemens H.M.\} and \{van Oers\}, \{Marinus H.J.\} and Keating, \{Michael J.\} and Alessandra Ferrajoli and Coombes, \{Kevin R.\} and Abruzzo, \{Lynne V.\} and Robetorye, \{Ryan S.\}",

note = "Funding Information: This work was supported in part by a grant from the CLL Global Research Foundation (L.V.A.). ",

year = "2009",

month = sep,

doi = "10.1016/j.leukres.2008.10.010",

language = "English (US)",

volume = "33",

pages = "1276--1281",

journal = "Leukemia Research",

issn = "0145-2126",

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number = "9",

}

TY - JOUR

T1 - Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene

AU - Gunn, Shelly R.

AU - Bolla, Aswani R.

AU - Barron, Lynn L.

AU - Gorre, Mercedes E.

AU - Mohammed, Mansoor S.

AU - Bahler, David W.

AU - Mellink, Clemens H.M.

AU - van Oers, Marinus H.J.

AU - Keating, Michael J.

AU - Ferrajoli, Alessandra

AU - Coombes, Kevin R.

AU - Abruzzo, Lynne V.

AU - Robetorye, Ryan S.

N1 - Funding Information: This work was supported in part by a grant from the CLL Global Research Foundation (L.V.A.).

PY - 2009/9

Y1 - 2009/9

N2 - We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to ∼1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.

AB - We used BAC array-based CGH to detect genomic imbalances in 187 CLL cases. Submicroscopic deletions of chromosome 22q11 were observed in 28 cases (15%), and the frequency of these deletions was second only to loss of the 13q14 region, the most common genomic aberration in CLL. Oligonucleotide-based array CGH analysis showed that the 22q11 deletions ranged in size from 0.34 Mb up to ∼1 Mb. The minimally deleted region included the ZNF280A, ZNF280B, GGTLC2, and PRAME genes. Quantitative real-time PCR revealed that ZNF280A, ZNF280B, and PRAME mRNA expression was significantly lower in the 22q11 deletion cases compared to non-deleted cases.

KW - Array CGH

KW - Chromosome 22q11

KW - Chronic lymphocytic leukemia

KW - PRAME gene

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UR - https://www.scopus.com/pages/publications/67549093672#tab=citedBy

U2 - 10.1016/j.leukres.2008.10.010

DO - 10.1016/j.leukres.2008.10.010

M3 - Article

C2 - 19027161

AN - SCOPUS:67549093672

SN - 0145-2126

VL - 33

SP - 1276

EP - 1281

JO - Leukemia Research

JF - Leukemia Research

IS - 9

ER -

Array CGH analysis of chronic lymphocytic leukemia reveals frequent cryptic monoallelic and biallelic deletions of chromosome 22q11 that include the PRAME gene

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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