Association between neuroendocrine tumors biomarkers and primary tumor site and disease type based on total ⁶⁸Ga-DOTATATE-avid tumor volume measurements

Objective: To determine the association between neuroendocrine tumor (NET) biomarker levels and the extent of disease as assessed by ⁶⁸Ga DOTATATE PET/CT imaging. Design: A retrospective analysis of a prospective database of patients with NETs. Methods: Fasting plasma chromogranin A (CgA), neuron-specific enolase (NSE), gastrin, glucagon, vasoactive intestinal peptide (VIP) and pancreatic polypeptide (PP), and 24-h urinary 5-hydroxyindoleacetic acid (5-HIAA) levels were measured. Correlation between biomarkers and total ⁶⁸Ga-DOTATATE-avid tumor volume (TV) was analyzed. Results: The analysis included 232 patients. In patients with pancreatic NETs (n = 112), ⁶⁸Ga-DOTATATE TV correlated with CgA (r = 0.6, P = 0.001, Spearman). In patients with multiple endocrine neoplasia type 1 (n = 39), 68Ga-DOTATATE TV correlated with glucagon (r = 0.5, P = 0.01) and PP levels (r = 0.5, P = 0.049). In patients with von Hippel-Lindau (n = 24), plasma VIP (r = 0.5, P = 0.02) and PP levels (r = 0.7, P < 0.001) correlated with ⁶⁸Ga-DOTATATE TV. In patients with small intestine NET (SINET, n = 74), ⁶⁸Ga-DOTATATE TV correlated with CgA (r = 0.5, P = 0.02) and 5-HIAA levels (r = 0.7, P < 0.001), with 5-HIAA >8.1 mg/24 h associated with metastatic disease with high positive (81.8%) and negative (85.7%) predictive values (P = 0.001). ⁶⁸Ga-DOTATATE TV in patients with NET of unknown primary (n = 16) and those with NET of other primary location (n = 30) correlated with 5-HIAA levels (r = 0.8, P = 0.002 and r = 0.7, P = 0.02 respectively). Conclusions: Our data supports the use of specific NET biomarkers based on the site of the primary NET and the presence of hereditary syndrome-associated NET. High urinary 5-HIAA levels indicate the presence of metastatic disease in patients with SINET.