@inbook{5583397d09cd4f1191c0453d4319d8a0,
title = "Association of glycolipids and growth factor receptors in lipid rafts",
abstract = "The traditional methods to study lipid rafts and their association with membrane proteins are based mainly on the isolation of a detergent-resistant membrane by biochemical fractionation. However, the use of detergents may induce lipid segregation and/or redistribution of membrane proteins during the process of sample preparation. Here, we describe a detergent-free method to study the glycolipid and growth factor receptor interaction and their association with lipid rafts. This method combines the biochemical and immunoblotting tools with confocal microscopic imaging, which allows for evaluation and verification of the membrane protein interaction and association with the lipid rafts components in a multifaceted manner.",
keywords = "Density gradient, Ganglioside, Growth factor receptor, Lipid raft, Neural stem cell",
author = "Jing Wang and Yu, {Robert K.}",
note = "Funding Information: This work was supported by a grant from NIH NS100896 to R.K.Y. and a grant from the American Heart Association 19CDA34490003 to J.W. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-0814-2_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "131--145",
booktitle = "Methods in Molecular Biology",
}