TY - JOUR
T1 - Association with the plasma membrane is sufficient for potentiating catalytic activity of regulators of g protein signaling (RGS) proteins of the R7 subfamily
AU - Muntean, Brian S.
AU - Martemyanov, Kirill A.
N1 - Funding Information:
This work was supported by National Institutes of Health Grants DA036596 and DA026405 (to K. A. M.). The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health
PY - 2016/3/25
Y1 - 2016/3/25
N2 - Regulators of G protein Signaling (RGS) promote deactivation of heterotrimeric G proteins thus controlling the magnitude and kinetics of responses mediated by G protein-coupled receptors (GPCR). In the nervous system, RGS7 and RGS9-2 play essential role in vision, reward processing, and movement control. Both RGS7 and RGS9-2 belong to the R7 subfamily of RGS proteins that form macromolecular complexes with R7-binding protein (R7BP). R7BP targets RGS proteins to the plasma membrane and augments their GTPase- Accelerating protein (GAP) activity, ultimately accelerating deactivation ofG protein signaling. However, it remains unclear if R7BP serves exclusively as a membrane anchoring subunit or further modulates RGS proteins to increase their GAP activity. To directly answer this question, we utilized a rapidly reversible chemically induced protein dimerization system that enabled us to control RGS localization independent from R7BP in living cells. To monitor kinetics of Gα deactivation, we coupled this strategy with measuring changes in theGAPactivity by bioluminescence resonance energy transfer-based assay in a cellular system containing α-opioid receptor. This approach was used to correlate changes in RGS localization and activity in the presence or absence of R7BP. Strikingly, we observed that RGS activity is augmented by membrane recruitment, in an orientation independent manner with no additional contributions provided by R7BP. These findings argue that the association of R7 RGS proteins with the membrane environment provides a major direct contribution to modulation of their GAP activity. copy; 2016 by The American Society for Biochemistry and Molecular Biology, Inc.
AB - Regulators of G protein Signaling (RGS) promote deactivation of heterotrimeric G proteins thus controlling the magnitude and kinetics of responses mediated by G protein-coupled receptors (GPCR). In the nervous system, RGS7 and RGS9-2 play essential role in vision, reward processing, and movement control. Both RGS7 and RGS9-2 belong to the R7 subfamily of RGS proteins that form macromolecular complexes with R7-binding protein (R7BP). R7BP targets RGS proteins to the plasma membrane and augments their GTPase- Accelerating protein (GAP) activity, ultimately accelerating deactivation ofG protein signaling. However, it remains unclear if R7BP serves exclusively as a membrane anchoring subunit or further modulates RGS proteins to increase their GAP activity. To directly answer this question, we utilized a rapidly reversible chemically induced protein dimerization system that enabled us to control RGS localization independent from R7BP in living cells. To monitor kinetics of Gα deactivation, we coupled this strategy with measuring changes in theGAPactivity by bioluminescence resonance energy transfer-based assay in a cellular system containing α-opioid receptor. This approach was used to correlate changes in RGS localization and activity in the presence or absence of R7BP. Strikingly, we observed that RGS activity is augmented by membrane recruitment, in an orientation independent manner with no additional contributions provided by R7BP. These findings argue that the association of R7 RGS proteins with the membrane environment provides a major direct contribution to modulation of their GAP activity. copy; 2016 by The American Society for Biochemistry and Molecular Biology, Inc.
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U2 - 10.1074/jbc.M115.713446
DO - 10.1074/jbc.M115.713446
M3 - Article
C2 - 26811338
AN - SCOPUS:84964817426
SN - 0021-9258
VL - 291
SP - 7195
EP - 7204
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 13
ER -