Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states

Qingqing Wei; Xiaoyang Cheng; Keying Chen; Jun Hu; Minqian Li; Peihong Zhu

doi:10.1360/02yc9025

Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states

Qingqing Wei, Xiaoyang Cheng, Keying Chen, Jun Hu, Minqian Li, Peihong Zhu

Research output: Contribution to journal › Article › peer-review

Abstract

Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca²⁺ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.

Original language	English (US)
Pages (from-to)	225-236
Number of pages	12
Journal	Science in China, Series C: Life Sciences
Volume	45
Issue number	3
DOIs	https://doi.org/10.1360/02yc9025
State	Published - 2002
Externally published	Yes

Keywords

Atomic force microscope
Elasticity
Ryanodine receptor/calcium release channel

ASJC Scopus subject areas

General Energy
General Environmental Science
Energy Engineering and Power Technology
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
Management, Monitoring, Policy and Law
Fuel Technology
Renewable Energy, Sustainability and the Environment
Modeling and Simulation

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10.1360/02yc9025

Cite this

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title = "Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states",

abstract = "Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca2+ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.",

keywords = "Atomic force microscope, Elasticity, Ryanodine receptor/calcium release channel",

author = "Qingqing Wei and Xiaoyang Cheng and Keying Chen and Jun Hu and Minqian Li and Peihong Zhu",

note = "Funding Information: Acknowledgements This work was supported by the Ninth Five-Year Major Research Programs of Chinese Academy of Sciences and the Major National Basic Research Program.",

year = "2002",

doi = "10.1360/02yc9025",

language = "English (US)",

volume = "45",

pages = "225--236",

journal = "Science in China, Series C: Life Sciences",

issn = "1006-9305",

publisher = "Science in China Press",

number = "3",

}

TY - JOUR

T1 - Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states

AU - Wei, Qingqing

AU - Cheng, Xiaoyang

AU - Chen, Keying

AU - Hu, Jun

AU - Li, Minqian

AU - Zhu, Peihong

N1 - Funding Information: Acknowledgements This work was supported by the Ninth Five-Year Major Research Programs of Chinese Academy of Sciences and the Major National Basic Research Program.

PY - 2002

Y1 - 2002

N2 - Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca2+ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.

AB - Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca2+ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.

KW - Atomic force microscope

KW - Elasticity

KW - Ryanodine receptor/calcium release channel

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DO - 10.1360/02yc9025

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C2 - 18759045

AN - SCOPUS:33646768160

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SP - 225

EP - 236

JO - Science in China, Series C: Life Sciences

JF - Science in China, Series C: Life Sciences

IS - 3

ER -

Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states

Abstract

Keywords

ASJC Scopus subject areas

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