Atrial natriuretic factor stimulates exocrine pancreatic secretion in the rat through NPR-C receptors

María E. Sabbatini, Alberto Villagra, Carlos A. Davio, Marcelo S. Vatta, Belisario E. Fernández, Liliana G. Bianciotti

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Increasing evidence supports the role of atrial natriuretic factor (ANF) in the modulation of gastrointestinal physiology. The effect of ANF on exocrine pancreatic secretion and the possible receptors and pathways involved were studied in vivo. Anesthetized rats were prepared with pancreatic duct cannulation, pyloric ligation, and bile diversion into the duodenum. ANF dose-dependently increased pancreatic secretion of fluid and proteins and enhanced secretin and CCK-evoked response. ANF decreased chloride secretion and increased the pH of the pancreatic juice. Neither cholinergic nor adrenergic blockade affected ANF-stimulated pancreatic secretion. Furthermore, ANF response was not mediated by the release of nitric oxide. ANF-evoked protein secretion was not inhibited by truncal vagotomy, atropine, or Nω-nitro-L-arginine methyl ester administration. The selective natriuretic peptide receptor-C (NPR-C) receptor agonist cANP-(4-23) mimicked ANF response in a dose-dependent fashion. When the intracellular signaling coupled to NPR-C receptors was investigated in isolated pancreatic acini, results showed that ANF did not modify basal or forskolin-evoked cAMP formation, but it dose-dependently enhanced phosphoinositide hydrolysis, which was blocked by the selective PLC inhibitor U-73122. ANF stimulated exocrine pancreatic secretion in the rat, and its effect was not mediated by nitric oxide or parasympathetic or sympathetic activity. Furthermore, CCK and secretin appear not to be involved in ANF response. Present findings support that ANF exerts a stimulatory effect on pancreatic exocrine secretion mediated by NPR-C receptors coupled to the phosphoinositide pathway.

Original languageEnglish (US)
Pages (from-to)G929-G937
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number5 48-5
StatePublished - Nov 2003
Externally publishedYes


  • Adenosine 3′,5′-cyclic monophosphate
  • Cholecystokinin
  • Nitric oxide
  • Phosphoinositide
  • Secretin

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)


Dive into the research topics of 'Atrial natriuretic factor stimulates exocrine pancreatic secretion in the rat through NPR-C receptors'. Together they form a unique fingerprint.

Cite this