Abstract
To study rolling of mouse neutrophils on P- and E-selectins in whole blood and without cell isolation, we constructed an autoperfused flow chamber made from rectangular microslides (0.2×2 mm) perfused from a carotid artery catheter. A differential pressure transducer served to measure wall shear stress. Green fluorescent neutrophils rolled on P-selectin but not E-selectin coated at 50 ng/ml, with some rolling on E-selectin at 150 ng/ml. However, when P- and E-selectins were coimmobilized, the resulting number of rolling neutrophils was sixfold and fourfold higher than on P- or E-selectin alone. Velocity and flux analysis shows that P-selectin initiates neutrophil rolling, and a small amount of E-selectin, unable to capture many neutrophils, reduces the rolling velocity of all neutrophils by more than 90%. The unexpected synergism between E- and P-selectins explains why neutrophil recruitment is enhanced when both selectins are expressed.
Original language | English (US) |
---|---|
Pages (from-to) | 985-993 |
Number of pages | 9 |
Journal | Journal of Leukocyte Biology |
Volume | 76 |
Issue number | 5 |
DOIs | |
State | Published - Nov 2004 |
Externally published | Yes |
Keywords
- Capture
- Leukocyte rolling
- Shear stress
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Cell Biology