Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P-selectin and E-selectin

Michael L. Smith, Markus Sperandio, Elena V. Galkina, Klaus Ley

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

To study rolling of mouse neutrophils on P- and E-selectins in whole blood and without cell isolation, we constructed an autoperfused flow chamber made from rectangular microslides (0.2×2 mm) perfused from a carotid artery catheter. A differential pressure transducer served to measure wall shear stress. Green fluorescent neutrophils rolled on P-selectin but not E-selectin coated at 50 ng/ml, with some rolling on E-selectin at 150 ng/ml. However, when P- and E-selectins were coimmobilized, the resulting number of rolling neutrophils was sixfold and fourfold higher than on P- or E-selectin alone. Velocity and flux analysis shows that P-selectin initiates neutrophil rolling, and a small amount of E-selectin, unable to capture many neutrophils, reduces the rolling velocity of all neutrophils by more than 90%. The unexpected synergism between E- and P-selectins explains why neutrophil recruitment is enhanced when both selectins are expressed.

Original languageEnglish (US)
Pages (from-to)985-993
Number of pages9
JournalJournal of Leukocyte Biology
Volume76
Issue number5
DOIs
StatePublished - Nov 2004
Externally publishedYes

Keywords

  • Capture
  • Leukocyte rolling
  • Shear stress

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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