TY - JOUR
T1 - Autoradiography of ganglioside antigens separated by high-performance. Thin-layer chromatography vith their antibodies
AU - Kasai, Noriyuki
AU - Naiki, Masaharu
AU - Yu, Robert K.
PY - 1984
Y1 - 1984
N2 - An improved method is described for the detection of ganglioside antigens with the antibodies on tbin-layer.chromatograms. The method involves the following steps: Separation of gangliosides on a high-performance thin-layer chrornatographic plate, application of antibody solution, and detection of bound antibody with [125]staphy-lococcal protein A. Using specific antibodies against gangliosides GM4, GM1, GD3, and asialo GM1 ganglioside, the method allowed positive identification of these antigens on thin-layer plates. It also provides a convenient means of assessing the specificity of an ant i-glycolipid antibody.
AB - An improved method is described for the detection of ganglioside antigens with the antibodies on tbin-layer.chromatograms. The method involves the following steps: Separation of gangliosides on a high-performance thin-layer chrornatographic plate, application of antibody solution, and detection of bound antibody with [125]staphy-lococcal protein A. Using specific antibodies against gangliosides GM4, GM1, GD3, and asialo GM1 ganglioside, the method allowed positive identification of these antigens on thin-layer plates. It also provides a convenient means of assessing the specificity of an ant i-glycolipid antibody.
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U2 - 10.1093/oxfordjournals.jbchem.a134821
DO - 10.1093/oxfordjournals.jbchem.a134821
M3 - Article
C2 - 6490603
AN - SCOPUS:0021457751
SN - 0021-924X
VL - 96
SP - 261
EP - 264
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 1
ER -