Beneficial effect of intracellular trehalose during mammalian cell storage

Tani Chen; Ali Eroglu; Alex Fowler; Stephen Cheley; Hagan Bayley; Mehmet Toner

Beneficial effect of intracellular trehalose during mammalian cell storage

Tani Chen, Ali Eroglu, Alex Fowler, Stephen Cheley, Hagan Bayley, Mehmet Toner

Research output: Contribution to journal › Conference article › peer-review

Abstract

Using a genetically engineered mutant of Staphylococcus aureus alpha-hemolysin to create pores in the cellular membrane, trehalose was successfully loaded into cells. Differential scanning calorimetry was used to study the glass transition temperature in cells containing intracellular trehalose. Results show higher glass transition temperatures and enhanced stability of the cytoplasm due to the presence of trehalose, compared to cells without intracellular trehalose. In addition, initial experiments show that cell survival depends on the glass transition temperature, as well as the storage temperature and the final moisture content.

Original language	English (US)
Journal	Annals of Biomedical Engineering
Volume	28
Issue number	SUPPL. 1
State	Published - Dec 1 2000
Externally published	Yes
Event	2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA Duration: Oct 12 2000 → Oct 14 2000

ASJC Scopus subject areas

Biomedical Engineering

Cite this

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title = "Beneficial effect of intracellular trehalose during mammalian cell storage",

abstract = "Using a genetically engineered mutant of Staphylococcus aureus alpha-hemolysin to create pores in the cellular membrane, trehalose was successfully loaded into cells. Differential scanning calorimetry was used to study the glass transition temperature in cells containing intracellular trehalose. Results show higher glass transition temperatures and enhanced stability of the cytoplasm due to the presence of trehalose, compared to cells without intracellular trehalose. In addition, initial experiments show that cell survival depends on the glass transition temperature, as well as the storage temperature and the final moisture content.",

author = "Tani Chen and Ali Eroglu and Alex Fowler and Stephen Cheley and Hagan Bayley and Mehmet Toner",

year = "2000",

month = dec,

day = "1",

language = "English (US)",

volume = "28",

journal = "Annals of Biomedical Engineering",

issn = "0090-6964",

publisher = "Springer Netherlands",

number = "SUPPL. 1",

note = "2000 Annual Fall Meeting of the Biomedical Engineering Society ; Conference date: 12-10-2000 Through 14-10-2000",

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TY - JOUR

T1 - Beneficial effect of intracellular trehalose during mammalian cell storage

AU - Chen, Tani

AU - Eroglu, Ali

AU - Fowler, Alex

AU - Cheley, Stephen

AU - Bayley, Hagan

AU - Toner, Mehmet

PY - 2000/12/1

Y1 - 2000/12/1

N2 - Using a genetically engineered mutant of Staphylococcus aureus alpha-hemolysin to create pores in the cellular membrane, trehalose was successfully loaded into cells. Differential scanning calorimetry was used to study the glass transition temperature in cells containing intracellular trehalose. Results show higher glass transition temperatures and enhanced stability of the cytoplasm due to the presence of trehalose, compared to cells without intracellular trehalose. In addition, initial experiments show that cell survival depends on the glass transition temperature, as well as the storage temperature and the final moisture content.

AB - Using a genetically engineered mutant of Staphylococcus aureus alpha-hemolysin to create pores in the cellular membrane, trehalose was successfully loaded into cells. Differential scanning calorimetry was used to study the glass transition temperature in cells containing intracellular trehalose. Results show higher glass transition temperatures and enhanced stability of the cytoplasm due to the presence of trehalose, compared to cells without intracellular trehalose. In addition, initial experiments show that cell survival depends on the glass transition temperature, as well as the storage temperature and the final moisture content.

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UR - http://www.scopus.com/inward/citedby.url?scp=0034507013&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0034507013

SN - 0090-6964

VL - 28

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

IS - SUPPL. 1

T2 - 2000 Annual Fall Meeting of the Biomedical Engineering Society

Y2 - 12 October 2000 through 14 October 2000

ER -

Beneficial effect of intracellular trehalose during mammalian cell storage

Abstract

ASJC Scopus subject areas

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