Biochemical Characteristics of Microbial Enzymes and Their Significance from Industrial Perspectives

Santosh Thapa, Hui Li, Joshua OHair, Sarabjit Bhatti, Fur Chi Chen, Kamal Al Nasr, Terrance Johnson, Suping Zhou

Research output: Contribution to journalReview articlepeer-review

60 Scopus citations


Microbes are ubiquitously distributed in nature and are a critical part of the holobiont fitness. They are perceived as the most potential biochemical reservoir of inordinately diverse and multi-functional enzymes. The robust nature of the microbial enzymes with thermostability, pH stability and multi-functionality make them potential candidates for the efficient biotechnological processes under diverse physio-chemical conditions. The need for sustainable solutions to various environmental challenges has further surged the demand for industrial enzymes. Fueled by the recent advent of recombinant DNA technology, genetic engineering, and high-throughput sequencing and omics techniques, numerous microbial enzymes have been developed and further exploited for various industrial and therapeutic applications. Most of the hydrolytic enzymes (protease being the dominant hydrolytic enzyme) have broad range of industrial uses such as food and feed processing, polymer synthesis, production of pharmaceuticals, manufactures of detergents, paper and textiles, and bio-fuel refinery. In this review article, after a short overview of microbial enzymes, an approach has been made to highlight and discuss their potential relevance in biotechnological applications and industrial bio-processes, significant biochemical characteristics of the microbial enzymes, and various tools that are revitalizing the novel enzymes discovery.

Original languageEnglish (US)
Pages (from-to)579-601
Number of pages23
JournalMolecular Biotechnology
Issue number8
StatePublished - Aug 1 2019
Externally publishedYes


  • Biochemical characteristics
  • Industrial applications
  • Microbial enzymes
  • Recombinant DNA technology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology


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