TY - JOUR
T1 - Biocompatible synthesis of peptide capped copper nanoparticles and their biological effect on tumor cells
AU - Valodkar, Mayur
AU - Jadeja, Ravirajsinh N.
AU - Thounaojam, Menaka C.
AU - Devkar, Ranjitsinh V.
AU - Thakore, Sonal
N1 - Funding Information:
The authors are grateful to the University Grants Commission , New Delhi for financial assistance. Special thanks to Dr. P.S. Nagar for providing Euphorbia nivulia latex for the investigation and Dr. Geeta S. Padate, Head, Department of Zoology for her encouragement.
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/7/15
Y1 - 2011/7/15
N2 - Extracellular synthesis of copper nanoparticles was carried out using stem latex of a medicinally important plant, Euphorbia nivulia. The nanoparticles were stabilized and subsequently capped by peptides and terpenoids present within the latex. The protein capping is a promising biocompatible vehicle for destruction of tumor/cancer cells. The cytotoxicity potential of the plant protein capped nanoparticles was evaluated using various parameters like MTT cell viability assay and extracellular lactate dehydrogenase (LDH) release in cancer cell line. Other parameters that determine the oxidative stress viz., reactive oxygen species (ROS) generation, intracellular reduced glutathione (GSH), malondialdehyde (MDA), superoxide generation and acridine orange/ethidium bromide staining were also investigated. The present study led to the conclusion that copper nanoparticles are toxic to A549 cells in a dose dependent manner. The non-toxic aqueous formulation of latex capped copper nanoparticles can be directly used for administration/in vivo delivery of nanoparticles for cancer therapy.
AB - Extracellular synthesis of copper nanoparticles was carried out using stem latex of a medicinally important plant, Euphorbia nivulia. The nanoparticles were stabilized and subsequently capped by peptides and terpenoids present within the latex. The protein capping is a promising biocompatible vehicle for destruction of tumor/cancer cells. The cytotoxicity potential of the plant protein capped nanoparticles was evaluated using various parameters like MTT cell viability assay and extracellular lactate dehydrogenase (LDH) release in cancer cell line. Other parameters that determine the oxidative stress viz., reactive oxygen species (ROS) generation, intracellular reduced glutathione (GSH), malondialdehyde (MDA), superoxide generation and acridine orange/ethidium bromide staining were also investigated. The present study led to the conclusion that copper nanoparticles are toxic to A549 cells in a dose dependent manner. The non-toxic aqueous formulation of latex capped copper nanoparticles can be directly used for administration/in vivo delivery of nanoparticles for cancer therapy.
KW - A. Biomaterials
KW - A. Nanostructures
KW - C. Electron microscopy
KW - C. Fourier transform infrared spectroscopy (FTIR)
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U2 - 10.1016/j.matchemphys.2011.02.039
DO - 10.1016/j.matchemphys.2011.02.039
M3 - Article
AN - SCOPUS:79955845543
SN - 0254-0584
VL - 128
SP - 83
EP - 89
JO - Materials Chemistry and Physics
JF - Materials Chemistry and Physics
IS - 1-2
ER -
