Blood volume measurement: The comparison of pulse dye densitometry and Dill and Costill's methods

Eric Belin De Chantemèle, Guillemette Gauquelin-Koch, Monique Duvareille, Natacha Pellet, Claude Gharib, Marc Antoine Custaud

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


In many clinical situations, it is crucial to determine circulating blood volume (BV) easily and to repeat this measurement. The Dye DensitoGram Analyzer® (DDG, Nihon Kohden Corp) measures semi-automatically BV, using an injection of IndoCyanine Green (ICG, 10 mg), and avoiding intermittent blood samples. The DDG was used during a 90-day microgravity simulation by Head-Down-Tilt bed rest (HDT) to measure BV and compared with the calculation of the plasma volume (PV) variations according to Dill and Costill's formula (DC). Seventeen healthy volunteers were included: 8 control subjects (Co) and 9 subjects submitted to a resistive exercise counter-measure (CM). Measurements were performed, one day before HDT, on days 3 and 90 of HDT and on day 9 after HDT. A double measurement of the BV was performed to assess the repeatability of this method. On the last day of HDT a significant decrease (p < 0.05) in the PV was noted with the DDG (Co: - 12.3 ± 5.7%, CM: - 9.0 ± 5.3%) and DC; (Co: - 4.7 ± 1.8%, CM: - 6.8 ± 2.5%). A good repeatability of the technique was shown with a low intrasubjects coefficient of variation (4.95 ± 0.95%) and an acceptable intersubjects coefficient of variation (15.30 ± 1.13%). No correlation was noted between DDG and DC (r 2 = 0.27). The DDG gives a good repeatability, not affected by the microgravity exposure. Thanks to its capacity to measure accurately the BV within 7-10 min, this device presents major advantages for clinical use and research purpose.

Original languageEnglish (US)
Pages (from-to)1564-1569
Number of pages6
JournalLife sciences
Issue number14
StatePublished - Feb 28 2006
Externally publishedYes


  • Blood volume
  • Dye DensitoGram Analyzer®
  • Indocyanine green
  • Microgravity simulation

ASJC Scopus subject areas

  • General Pharmacology, Toxicology and Pharmaceutics
  • General Biochemistry, Genetics and Molecular Biology


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