TY - JOUR
T1 - Blood volume measurement
T2 - The comparison of pulse dye densitometry and Dill and Costill's methods
AU - Belin De Chantemèle, Eric
AU - Gauquelin-Koch, Guillemette
AU - Duvareille, Monique
AU - Pellet, Natacha
AU - Gharib, Claude
AU - Custaud, Marc Antoine
N1 - Funding Information:
The authors acknowledge CNES (Centre National d'Etudes Spatiales), ESA (European Space Agency) and NASDA (National Space Development Agency of Japan) for their financial support during this study.
PY - 2006/2/28
Y1 - 2006/2/28
N2 - In many clinical situations, it is crucial to determine circulating blood volume (BV) easily and to repeat this measurement. The Dye DensitoGram Analyzer® (DDG, Nihon Kohden Corp) measures semi-automatically BV, using an injection of IndoCyanine Green (ICG, 10 mg), and avoiding intermittent blood samples. The DDG was used during a 90-day microgravity simulation by Head-Down-Tilt bed rest (HDT) to measure BV and compared with the calculation of the plasma volume (PV) variations according to Dill and Costill's formula (DC). Seventeen healthy volunteers were included: 8 control subjects (Co) and 9 subjects submitted to a resistive exercise counter-measure (CM). Measurements were performed, one day before HDT, on days 3 and 90 of HDT and on day 9 after HDT. A double measurement of the BV was performed to assess the repeatability of this method. On the last day of HDT a significant decrease (p < 0.05) in the PV was noted with the DDG (Co: - 12.3 ± 5.7%, CM: - 9.0 ± 5.3%) and DC; (Co: - 4.7 ± 1.8%, CM: - 6.8 ± 2.5%). A good repeatability of the technique was shown with a low intrasubjects coefficient of variation (4.95 ± 0.95%) and an acceptable intersubjects coefficient of variation (15.30 ± 1.13%). No correlation was noted between DDG and DC (r 2 = 0.27). The DDG gives a good repeatability, not affected by the microgravity exposure. Thanks to its capacity to measure accurately the BV within 7-10 min, this device presents major advantages for clinical use and research purpose.
AB - In many clinical situations, it is crucial to determine circulating blood volume (BV) easily and to repeat this measurement. The Dye DensitoGram Analyzer® (DDG, Nihon Kohden Corp) measures semi-automatically BV, using an injection of IndoCyanine Green (ICG, 10 mg), and avoiding intermittent blood samples. The DDG was used during a 90-day microgravity simulation by Head-Down-Tilt bed rest (HDT) to measure BV and compared with the calculation of the plasma volume (PV) variations according to Dill and Costill's formula (DC). Seventeen healthy volunteers were included: 8 control subjects (Co) and 9 subjects submitted to a resistive exercise counter-measure (CM). Measurements were performed, one day before HDT, on days 3 and 90 of HDT and on day 9 after HDT. A double measurement of the BV was performed to assess the repeatability of this method. On the last day of HDT a significant decrease (p < 0.05) in the PV was noted with the DDG (Co: - 12.3 ± 5.7%, CM: - 9.0 ± 5.3%) and DC; (Co: - 4.7 ± 1.8%, CM: - 6.8 ± 2.5%). A good repeatability of the technique was shown with a low intrasubjects coefficient of variation (4.95 ± 0.95%) and an acceptable intersubjects coefficient of variation (15.30 ± 1.13%). No correlation was noted between DDG and DC (r 2 = 0.27). The DDG gives a good repeatability, not affected by the microgravity exposure. Thanks to its capacity to measure accurately the BV within 7-10 min, this device presents major advantages for clinical use and research purpose.
KW - Blood volume
KW - Dye DensitoGram Analyzer®
KW - Indocyanine green
KW - Microgravity simulation
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U2 - 10.1016/j.lfs.2005.07.025
DO - 10.1016/j.lfs.2005.07.025
M3 - Article
C2 - 16260003
AN - SCOPUS:32944456628
SN - 0024-3205
VL - 78
SP - 1564
EP - 1569
JO - Life sciences
JF - Life sciences
IS - 14
ER -