Blue light generates reactive oxygen species (ROS) differentially in tumor vs. normal epithelial cells

Daniel B. Lockwood; John C. Wataha; Jill B. Lewis; Wan Y. Tseng; Regina L.W. Messer; Stephen D. Hsu

doi:10.1016/j.dental.2004.07.022

Blue light generates reactive oxygen species (ROS) differentially in tumor vs. normal epithelial cells

Daniel B. Lockwood, John C. Wataha, Jill B. Lewis, Wan Y. Tseng, Regina L.W. Messer, Stephen D. Hsu

Oral Biology & Dx Sciences

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

The use of blue light of high intensity in dentistry to activate polymerization of resin restorative materials was analyzed. Intracellular reactive oxygen species (ROS) were estimated using dihydrofluorescein diacetate (HFLUOR-DA), a vital fluorescein-based redox sensitive dye. It was found that if the biological effects of blue light are redox-mediated, antioxidants might be used to mitigate unwanted side effects of blue light during clinical use. Dose-dependent ROS levels were generated in NHEK and OSC2 cells. It was also found that both vitamin E and NAC significantly reduced blue-light-induced levels of ROS, and were more effective in the OSC2 cells.

Original language	English (US)
Pages (from-to)	683-688
Number of pages	6
Journal	Dental Materials
Volume	21
Issue number	7
DOIs	https://doi.org/10.1016/j.dental.2004.07.022
State	Published - Jul 2005

Keywords

Antioxidants
Epithelium
Fluorescein
ROS
Vitamin E

ASJC Scopus subject areas

Materials Science(all)
Dentistry(all)
Mechanics of Materials

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10.1016/j.dental.2004.07.022

Cite this

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title = "Blue light generates reactive oxygen species (ROS) differentially in tumor vs. normal epithelial cells",

abstract = "The use of blue light of high intensity in dentistry to activate polymerization of resin restorative materials was analyzed. Intracellular reactive oxygen species (ROS) were estimated using dihydrofluorescein diacetate (HFLUOR-DA), a vital fluorescein-based redox sensitive dye. It was found that if the biological effects of blue light are redox-mediated, antioxidants might be used to mitigate unwanted side effects of blue light during clinical use. Dose-dependent ROS levels were generated in NHEK and OSC2 cells. It was also found that both vitamin E and NAC significantly reduced blue-light-induced levels of ROS, and were more effective in the OSC2 cells.",

keywords = "Antioxidants, Epithelium, Fluorescein, ROS, Vitamin E",

author = "Lockwood, {Daniel B.} and Wataha, {John C.} and Lewis, {Jill B.} and Tseng, {Wan Y.} and Messer, {Regina L.W.} and Hsu, {Stephen D.}",

note = "Funding Information: The authors thank the Medical College of Georgia School of Dentistry Biocompatibility program for partial support of this work. ",

year = "2005",

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doi = "10.1016/j.dental.2004.07.022",

language = "English (US)",

volume = "21",

pages = "683--688",

journal = "Dental Materials",

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publisher = "Elsevier Science",

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T1 - Blue light generates reactive oxygen species (ROS) differentially in tumor vs. normal epithelial cells

AU - Lockwood, Daniel B.

AU - Wataha, John C.

AU - Lewis, Jill B.

AU - Tseng, Wan Y.

AU - Messer, Regina L.W.

AU - Hsu, Stephen D.

N1 - Funding Information: The authors thank the Medical College of Georgia School of Dentistry Biocompatibility program for partial support of this work.

PY - 2005/7

Y1 - 2005/7

N2 - The use of blue light of high intensity in dentistry to activate polymerization of resin restorative materials was analyzed. Intracellular reactive oxygen species (ROS) were estimated using dihydrofluorescein diacetate (HFLUOR-DA), a vital fluorescein-based redox sensitive dye. It was found that if the biological effects of blue light are redox-mediated, antioxidants might be used to mitigate unwanted side effects of blue light during clinical use. Dose-dependent ROS levels were generated in NHEK and OSC2 cells. It was also found that both vitamin E and NAC significantly reduced blue-light-induced levels of ROS, and were more effective in the OSC2 cells.

AB - The use of blue light of high intensity in dentistry to activate polymerization of resin restorative materials was analyzed. Intracellular reactive oxygen species (ROS) were estimated using dihydrofluorescein diacetate (HFLUOR-DA), a vital fluorescein-based redox sensitive dye. It was found that if the biological effects of blue light are redox-mediated, antioxidants might be used to mitigate unwanted side effects of blue light during clinical use. Dose-dependent ROS levels were generated in NHEK and OSC2 cells. It was also found that both vitamin E and NAC significantly reduced blue-light-induced levels of ROS, and were more effective in the OSC2 cells.

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KW - Epithelium

KW - Fluorescein

KW - ROS

KW - Vitamin E

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JO - Dental Materials

JF - Dental Materials

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ER -

Blue light generates reactive oxygen species (ROS) differentially in tumor vs. normal epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

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