Bone generation in the reconstruction of a critical size calvarial defect in an experimental model

Yong Chen Por, C. Raul Barceló, Kenneth E. Salyer, David G. Genecov, Karen Troxel, El Gendler, Mohammed Elsayed Elsalanty, Lynne A. Opperman

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


This study was designed to investigate the optimal combination of known osteogenic biomaterials with shape conforming struts to achieve calvarial vault reconstruction, using a canine model. Eighteen adolescent beagles were divided equally into 6 groups. A critical-size defect of 6 x 2 cm traversed the sagittal suture. The biomaterials used for calvarial reconstruction were demineralized perforated bone matrix (DBM), recombinant human bone morphogenetic protein 2 (rhBMP2), and autogenous platelet-rich plasma (PRP). The struts used were cobalt chrome (metal) or resorbable plate. The groupings were as follows: 1) DBM + metal, 2) DBM + PRP + metal, 3) DBM + PRP + resorbable plate, 4) DBM + rhBMP2 + metal, 5) DBM + rhBMP2 + PRP + metal, and 6) DBM + rhBMP2 + resorbable plate. Animals were killed at 3 months after surgery. There was no mortality or major complications. Analysis was performed macroscopically and histologically and with computed tomography. There was complete bony regeneration in the rhBMP2 groups only. Non-rhBMP2 groups had minimal bony ingrowth from the defect edges and on the dural surface, a finding confirmed by computed tomographic scan and histology. Platelet-rich plasma did not enhance bone regeneration. Shape conformation was good with both metal and resorbable plate. rhBMP2, but not PRP, accelerated calvarial regeneration in 3 months. The DBMs in the rhBMP2 groups were substituted by new trabecular bone. Shape molding was good with both metal and resorbable plate.

Original languageEnglish (US)
Pages (from-to)383-392
Number of pages10
JournalJournal of Craniofacial Surgery
Issue number2
StatePublished - Mar 1 2008


  • Cranial vault reconstruction
  • Critical size calvarial defect
  • Metal struts
  • Resorbable plates
  • rhBMP2

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology


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