Bone generation in the reconstruction of a critical size calvarial defect in an experimental model

Yong Chen Por, Carlos Raul Barceló, Kenneth E. Salyer, David G. Genecov, Karen Troxel, El Gendler, Mohammed Elsayed Elsalanty, Lynne A. Opperman

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Objective: This study was designed to investigate the optimal combination of known osteogenic biomaterials with shape conforming struts to achieve calvarial vault reconstruction, using a canine model. Methods: Eighteen adolescent beagles were divided equally into 6 groups. A critical size defect of 6 x 2 cm traversed the sagittal suture. The biomaterials used for calvarial reconstruction were demineralised perforated bone matrix (DBM), recombinant human bone morphogenetic protein-2 (rhBMP2) and autogenous platelet-rich plasma (PRP). The struts used were cobalt chrome (metal) or resorbable plate. The groupings were as follows: 1) DBM + metal, 2) DBM + PRP + metal, 3) DBM + PRP + resorbable plate, 4) DBM + rhBMP2 + metal, 5) DBM + rhBMP2 + PRP + metal, and 6) DBM + rhBMP2 + resorbable plate. Animals were euthanised at 3 months post-surgery. There was no mortality or major complications. Analysis was performed macroscopically, histologically, and with computed tomography (CT). Results: There was complete bony regeneration in the rhBMP2 groups only. Non-rhBMP2 groups had minimal bony ingrowth from the defect edges and on the dural surface, a finding confirmed by CT scan and histology. PRP did not enhance bone regeneration. Shape conformation was good with both metal and resorbable plate. Conclusion: rhBMP2 but not PRP accelerated calvarial regeneration in 3 months. The DBM in the rhBMP2 groups were substituted by new trabecular bone. Shape molding was good with both metal and resorbable plate.

Original languageEnglish (US)
Pages (from-to)911-919
Number of pages9
JournalAnnals of the Academy of Medicine Singapore
Issue number11
StatePublished - Nov 1 2007
Externally publishedYes


  • Cranial vault reconstruction
  • Critical size calvarial defect
  • Metal struts
  • Resorbable plates
  • rhBMP2

ASJC Scopus subject areas

  • Medicine(all)


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