TY - JOUR
T1 - Boron nitride nanotubes and layer-by-layer polyelectrolyte coating for yeast cell surface engineering
AU - Emanet, Melis
AU - Fakhrullin, Rawil
AU - Çulha, Mustafa
N1 - Funding Information:
The authors acknowledge the financial support of the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 12M480) and Yeditepe University. This work was partially funded by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities (RF).
Funding Information:
The authors acknowledge the financial support of the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 112M480) and Yeditepe University. This work was partially funded by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities (RF).
Publisher Copyright:
© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - The application of living microbial cells as molecular engines for a variety of biotechnological applications including cell-based biosensing is an ongoing research effort. However, there are significant difficulties to overcome such as the fragile structures of microbial cells and the weak efficiency of developed systems for detecting toxic agents in the environment. In this Communication, we demonstrate the interfacing of hydroxylated boron nitride nanotubes (BNNT-OHs) with live yeast cell surfaces. BNNT-OHs were incorporated with polyelectrolytes (PEs) using layer-by-layer deposition onto live Saccharomyces cerevisiae cells. The PE- and BNNT-OH-coated yeast was characterized using spectroscopic and imaging techniques (dynamic light scattering, FTIR, and SEM). Importantly, BNNT-OH-coated yeast cells were viable after the surface modification with nanotubes. We believe that BNNT-OHs-functionalized yeast will find numerous applications in biotechnology.
AB - The application of living microbial cells as molecular engines for a variety of biotechnological applications including cell-based biosensing is an ongoing research effort. However, there are significant difficulties to overcome such as the fragile structures of microbial cells and the weak efficiency of developed systems for detecting toxic agents in the environment. In this Communication, we demonstrate the interfacing of hydroxylated boron nitride nanotubes (BNNT-OHs) with live yeast cell surfaces. BNNT-OHs were incorporated with polyelectrolytes (PEs) using layer-by-layer deposition onto live Saccharomyces cerevisiae cells. The PE- and BNNT-OH-coated yeast was characterized using spectroscopic and imaging techniques (dynamic light scattering, FTIR, and SEM). Importantly, BNNT-OH-coated yeast cells were viable after the surface modification with nanotubes. We believe that BNNT-OHs-functionalized yeast will find numerous applications in biotechnology.
KW - Cell surface engineering
KW - Cell-nanotube interfacing
KW - Hydroxylated boron nitride nanotubes
KW - Layer-bylayer deposition
KW - Saccharomyces cerevisiae
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U2 - 10.1002/cnma.201600044
DO - 10.1002/cnma.201600044
M3 - Article
AN - SCOPUS:85016389354
SN - 2199-692X
VL - 2
SP - 426
EP - 429
JO - ChemNanoMat
JF - ChemNanoMat
IS - 5
ER -
