Brain-derived estrogen exerts anti-inflammatory and neuroprotective actions in the rat hippocampus

Quanguang Zhang, Ruimin Wang, Hui Tang, Yan Dong, Alice Chan, Gangadhara Reddy Sareddy, Ratna K. Vadlamudi, Darrell W Brann

Research output: Contribution to journalArticlepeer-review

99 Scopus citations


17β-estradiol (E2) has been implicated to play a critical role in neuroprotection, synaptic plasticity, and cognitive function. Classically, the role of gonadal-derived E2 in these events is well established, but the role of brain-derived E2 is less clear. To address this issue, we investigated the expression, localization, and modulation of aromatase and local E2 levels in the hippocampus following global cerebral ischemia (GCI) in adult ovariectomized rats. Immunohistochemistry (IHC) revealed that the hippocampal regions CA1, CA3 and dentate gyrus (DG) exhibited high levels of immunoreactive aromatase staining, with aromatase being co-localized primarily in neurons in non-ischemic animals. Following GCI, aromatase became highly expressed in GFAP-positive astrocytes in the hippocampal CA1 region at 2-3. days post GCI reperfusion. An ELISA for E2 and IHC for E2 confirmed the GCI-induced elevation of local E2 in the CA1 region and that the increase in local E2 occurred in astrocytes. Furthermore, central administration of aromatase antisense (AS) oligonucleotides, but not missense (MS) oligonucleotides, blocked the increase in aromatase and local E2 in astrocytes after GCI, and resulted in a significant increase in GCI-induced hippocampal CA1 region neuronal cell death and neuroinflammation. As a whole, these results suggest that brain-derived E2 exerts important neuroprotective and anti-inflammatory actions in the hippocampal CA1 region following GCI.

Original languageEnglish (US)
Pages (from-to)84-91
Number of pages8
JournalMolecular and Cellular Endocrinology
Issue number1-2
StatePublished - May 25 2014


  • Aromatase
  • Brain
  • Estrogen
  • Hippocampus
  • Neuroprotection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology


Dive into the research topics of 'Brain-derived estrogen exerts anti-inflammatory and neuroprotective actions in the rat hippocampus'. Together they form a unique fingerprint.

Cite this