Carbodiimide inactivation of matrix metalloproteinases in radicular dentine

Allegra Comba, Nicola Scotti, Annalisa Mazzoni, Tatjana Maravic, Sandra Ribeiro Cunha, Riccardo Michelotto Tempesta, Massimo Carossa, David H. Pashley, Franklin Chi Meng Tay, Lorenzo Breschi

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Objectives: The present in vitro study evaluated the effect of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), a cross-linking agent used as an additional therapeutic primer for luting fiber posts to radicular dentine to prevent hybrid layer degradation. Methods: Root canal treatment was performed on 80 extracted single-rooted human teeth. A 10-mm post space was prepared and pecimens were randomly assigned to four groups (n=20) according to the bonding system: 1) All Bond 3 (Bisco); 2) All Bond 3 + 0.3M EDC; 3) Prime&Bond XP (Dentsply Sirona); 4) Prime&Bond XP + 0.3M EDC. In groups 2 and 4, EDC was applied on phosphoric acid-etched dentine for 1 min. Fiber posts (RelyX Fiber Post, 3M ESPE) were luted with a dual-cured resin cement (Core-X flow, Dentsply Sirona). Slices were prepared for micro push-out test and interfacial nanoleakage evaluation of the coronal and apical region of the canal space after 24 h and 1 year storage in artificial saliva. In-situ zymography was performed to investigate endogenous matrix metalloproteinase activities within the hybrid layer. Results were statistically analysed with three-way ANOVA test or Chi Square test. Statistical significance was set at α=0.05. Results: No significant influence was identified between the two adhesives. The use of EDC significantly improved fiber post bond strength at 1 year but not at 24 h. Application of 0.3 M EDC prior to bonding significantly reduced gelatinolytic activities within the radicular hybrid layers. Conclusions: Carbodiimide was effective in preserving fibre post bond strength over time, through reducing the activities of intra-radicular endogenous proteases. Clinical Significance: Inhibition of matrix metalloproteinases using EDC over radicular dentin could play an important role in bond strength preservation. However, the clinical relevance of these findings needs to be proven.

Original languageEnglish (US)
Pages (from-to)56-62
Number of pages7
JournalJournal of Dentistry
StatePublished - Mar 2019


  • Carbodiimide
  • In situ zymography
  • Matrix metalloproteinases
  • Push-out

ASJC Scopus subject areas

  • Dentistry(all)


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