Abstract
A simple high performance-liquid chromatographic procedure employing electrochemical detection (LC-EC) has been developed for the quantitation of the cardiotonic agent dobutamine and a new pharmacologically active congener. The drugs were extracted from plasma by adsorption on to alumina, eluted from the adsorbent with dilute perchloric acid and the eluate subjected to LC-EC. The method had a limit of sensitivity of 1 ng/ml of plasma for each drug. The applicability of the LC-EC procedure was demonstrated by determining steady-state plasma concentration profiles of dobutamine and its congener in man and dog, respectively, following i.v. infusion of the drugs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 313-316 |
| Number of pages | 4 |
| Journal | Research Communications in Chemical Pathology and Pharmacology |
| Volume | 48 |
| Issue number | 2 |
| State | Published - 1985 |
| Externally published | Yes |
ASJC Scopus subject areas
- General Pharmacology, Toxicology and Pharmaceutics
- Pathology and Forensic Medicine
- Toxicology
- Pharmacology