Carrier-mediated transport of glycyl-L-proline in renal brush border vesicles

Vadivel Ganapathy; Joseph Mendicino; David H. Pashley; Frederick H. Leibach

doi:10.1016/0006-291X(80)91493-X

Carrier-mediated transport of glycyl-L-proline in renal brush border vesicles

Vadivel Ganapathy, Joseph Mendicino, David H. Pashley, Frederick H. Leibach

Oral Biology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Renal brush border vesicles prepared from rabbit were shown to transport intact glycyl-L-proline into the intravesicular space by a Na⁺-independent, carrier-mediated process. The kinetics of hydrolysis of glycyl-L-proline by the vesicles showed that this dipeptide was extremely resistant to hydrolysis. The intravesicular concentration of glycyl-L-proline was the same in both NaCl and KCl media. Dipeptide uptake into the vesicles appeared to be via carrier-mediated transport down a concentration gradient.

Original language	English (US)
Pages (from-to)	1133-1139
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	97
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(80)91493-X
State	Published - Dec 16 1980

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Carrier-mediated transport of glycyl-L-proline in renal brush border vesicles",

abstract = "Renal brush border vesicles prepared from rabbit were shown to transport intact glycyl-L-proline into the intravesicular space by a Na+-independent, carrier-mediated process. The kinetics of hydrolysis of glycyl-L-proline by the vesicles showed that this dipeptide was extremely resistant to hydrolysis. The intravesicular concentration of glycyl-L-proline was the same in both NaCl and KCl media. Dipeptide uptake into the vesicles appeared to be via carrier-mediated transport down a concentration gradient.",

author = "Vadivel Ganapathy and Joseph Mendicino and Pashley, {David H.} and Leibach, {Frederick H.}",

note = "Funding Information: XJe would like to thank Miss Lisa Brooks for her secretarial assistance. This study was supported in part by Research Grant AM 13150 from the National Institutes of Health, and USPHS-NIH Grant FR 5365.",

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AU - Mendicino, Joseph

AU - Pashley, David H.

AU - Leibach, Frederick H.

N1 - Funding Information: XJe would like to thank Miss Lisa Brooks for her secretarial assistance. This study was supported in part by Research Grant AM 13150 from the National Institutes of Health, and USPHS-NIH Grant FR 5365.

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N2 - Renal brush border vesicles prepared from rabbit were shown to transport intact glycyl-L-proline into the intravesicular space by a Na+-independent, carrier-mediated process. The kinetics of hydrolysis of glycyl-L-proline by the vesicles showed that this dipeptide was extremely resistant to hydrolysis. The intravesicular concentration of glycyl-L-proline was the same in both NaCl and KCl media. Dipeptide uptake into the vesicles appeared to be via carrier-mediated transport down a concentration gradient.

AB - Renal brush border vesicles prepared from rabbit were shown to transport intact glycyl-L-proline into the intravesicular space by a Na+-independent, carrier-mediated process. The kinetics of hydrolysis of glycyl-L-proline by the vesicles showed that this dipeptide was extremely resistant to hydrolysis. The intravesicular concentration of glycyl-L-proline was the same in both NaCl and KCl media. Dipeptide uptake into the vesicles appeared to be via carrier-mediated transport down a concentration gradient.

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Carrier-mediated transport of glycyl-L-proline in renal brush border vesicles

Abstract

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