Ca2+ imaging as a tool to assess TRP channel function in murine distal nephrons

Mykola Mamenko, Oleg Zaika, Roger G. O'Neil, Oleh Pochynyuk

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations


Transient receptor potential (TRP) channels are expressed in almost every segment of renal nephron from the glomerulus to the inner medullary collecting duct. Serving as a route for Ca2+ entry from the intratubular space into cells in response to external cues, TRP channels modulate water-electrolyte transport, thus determining functional properties of the renal tubule. In this chapter, we discuss technical aspects of using Ca2+ imaging to monitor activity of TRP channels in situ, namely, in the freshly isolated distal nephrons, with a special emphasis on the mechanosensitive TRPV4 channel and its role in tubular flow sensing.

Original languageEnglish (US)
Title of host publicationIon Channels
Subtitle of host publicationMethods and Protocols
EditorsNikita Gamper
Number of pages14
StatePublished - May 8 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Collecting duct
  • Connecting tubule
  • Fura-2
  • Mechanosensitivity
  • Shear stress

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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