@inbook{550f7aab72e849e097225a4c23d5ad5d,
title = "Ca2+ imaging as a tool to assess TRP channel function in murine distal nephrons",
abstract = "Transient receptor potential (TRP) channels are expressed in almost every segment of renal nephron from the glomerulus to the inner medullary collecting duct. Serving as a route for Ca2+ entry from the intratubular space into cells in response to external cues, TRP channels modulate water-electrolyte transport, thus determining functional properties of the renal tubule. In this chapter, we discuss technical aspects of using Ca2+ imaging to monitor activity of TRP channels in situ, namely, in the freshly isolated distal nephrons, with a special emphasis on the mechanosensitive TRPV4 channel and its role in tubular flow sensing.",
keywords = "Collecting duct, Connecting tubule, Fura-2, Mechanosensitivity, Shear stress",
author = "Mykola Mamenko and Oleg Zaika and O'Neil, {Roger G.} and Oleh Pochynyuk",
year = "2013",
month = may,
day = "8",
doi = "10.1007/978-1-62703-351-0_29",
language = "English (US)",
isbn = "9781627033503",
series = "Methods in Molecular Biology",
pages = "371--384",
editor = "Nikita Gamper",
booktitle = "Ion Channels",
}