TY - JOUR
T1 - cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome
AU - Saito, Akihiko
AU - Watanabe, Takeshi K.
AU - Shimada, Yoshikazu
AU - Fujiwara, Tsutomu
AU - Slaughter, Clive A.
AU - Demartino, George N.
AU - Tanahashi, Nobuyuki
AU - Tanaka, Keiji
N1 - Funding Information:
This work was supported in part by grants from the program Grant-in-Aid for Scientific Research on Priority Areas (intracellular proteolysis) from the Monbusho of Japan (K.T.) and the Human Frontier Science Promotion Organization (K.T. and G.N.D.).
PY - 1997/12/12
Y1 - 1997/12/12
N2 - We have employed cDNA cloning to deduce the complete primary structures of p44.5 and p55, two subunits of PA700, a 700-kDa multisubunit regulatory complex of the human 26S proteasome. These polypeptides consist of 422 and 456 amino acids with calculated molecular masses of 47,463 and 52,903, and isoelectric points of 6.06 and 7.56, respectively. Computer-assisted homology analysis revealed high sequence similarities of p44.5 and p55 with yeast proteins whose functions are yet unknown. Disruption of the yeast genes, termed NAS4 and NAS5 (non-ATPase subunits 4 and 5), resulted in lethality, indicating that each of the two subunits is essential for proliferation of yeast cells.
AB - We have employed cDNA cloning to deduce the complete primary structures of p44.5 and p55, two subunits of PA700, a 700-kDa multisubunit regulatory complex of the human 26S proteasome. These polypeptides consist of 422 and 456 amino acids with calculated molecular masses of 47,463 and 52,903, and isoelectric points of 6.06 and 7.56, respectively. Computer-assisted homology analysis revealed high sequence similarities of p44.5 and p55 with yeast proteins whose functions are yet unknown. Disruption of the yeast genes, termed NAS4 and NAS5 (non-ATPase subunits 4 and 5), resulted in lethality, indicating that each of the two subunits is essential for proliferation of yeast cells.
KW - Gene disruption
KW - Non-ATPase subunit
KW - PA700
KW - Ubiquitin
KW - Yeast
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U2 - 10.1016/S0378-1119(97)00524-6
DO - 10.1016/S0378-1119(97)00524-6
M3 - Article
C2 - 9426256
AN - SCOPUS:0030856132
SN - 0378-1119
VL - 203
SP - 241
EP - 250
JO - Gene
JF - Gene
IS - 2
ER -