Ceramide-induced vasorelaxation: An inhibitory action on protein kinase C

Douglas G. Johns; Jong Shiaw Jin; Dixon W. Wilde; R. Clinton Webb

doi:10.1016/S0306-3623(99)00038-5

Ceramide-induced vasorelaxation: An inhibitory action on protein kinase C

Douglas G. Johns, Jong Shiaw Jin, Dixon W. Wilde, R. Clinton Webb

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Experiments were designed to examine the role of sphingosine, PP2A phosphatases, and protein kinase C (PKC) inhibition in mediating the vasodilatory effects of ceramide in rat thoracic aorta. Sphingosine did not cause vasorelaxation, and oleoylethanolamine, a ceramidase inhibitor, did not affect sphingomyelinase-induced relaxation. Okadaic acid potentiated the relaxation response to ceramide. These observations rule out involvement of sphingosine and PP2A phosphatases in mediating ceramide-induced relaxation. Sphingomyelinase attenuated contractile and single-cell intracellular calcium responses to phorbol ester. Chelerythrine incubation potentiated the relaxation response to ceramide. These observations support a role for PKC inhibition in mediating the vasodilatory effects of ceramide. Copyright (C) 1999 Elsevier Science Inc.

Original language	English (US)
Pages (from-to)	415-421
Number of pages	7
Journal	General Pharmacology
Volume	33
Issue number	5
DOIs	https://doi.org/10.1016/S0306-3623(99)00038-5
State	Published - Nov 1999
Externally published	Yes

Keywords

Ceramide
Okadaic acid
Oleoylethanolamine
Sphingosine
Vascular smooth muscle
Vasorelaxation

ASJC Scopus subject areas

Pharmacology

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10.1016/S0306-3623(99)00038-5

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title = "Ceramide-induced vasorelaxation: An inhibitory action on protein kinase C",

abstract = "Experiments were designed to examine the role of sphingosine, PP2A phosphatases, and protein kinase C (PKC) inhibition in mediating the vasodilatory effects of ceramide in rat thoracic aorta. Sphingosine did not cause vasorelaxation, and oleoylethanolamine, a ceramidase inhibitor, did not affect sphingomyelinase-induced relaxation. Okadaic acid potentiated the relaxation response to ceramide. These observations rule out involvement of sphingosine and PP2A phosphatases in mediating ceramide-induced relaxation. Sphingomyelinase attenuated contractile and single-cell intracellular calcium responses to phorbol ester. Chelerythrine incubation potentiated the relaxation response to ceramide. These observations support a role for PKC inhibition in mediating the vasodilatory effects of ceramide. Copyright (C) 1999 Elsevier Science Inc.",

keywords = "Ceramide, Okadaic acid, Oleoylethanolamine, Sphingosine, Vascular smooth muscle, Vasorelaxation",

author = "Johns, {Douglas G.} and Jin, {Jong Shiaw} and Wilde, {Dixon W.} and Webb, {R. Clinton}",

note = "Funding Information: The authors wish to thank Deborah Brozovich and Timothy Yoon for their technical assistance. This study was supported by grants from the National Institutes of Health (HL-18575, GM-07767).",

year = "1999",

month = nov,

doi = "10.1016/S0306-3623(99)00038-5",

language = "English (US)",

volume = "33",

pages = "415--421",

journal = "General Pharmacology",

issn = "0306-3623",

publisher = "Elsevier Inc.",

number = "5",

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TY - JOUR

T1 - Ceramide-induced vasorelaxation

T2 - An inhibitory action on protein kinase C

AU - Johns, Douglas G.

AU - Jin, Jong Shiaw

AU - Wilde, Dixon W.

AU - Webb, R. Clinton

N1 - Funding Information: The authors wish to thank Deborah Brozovich and Timothy Yoon for their technical assistance. This study was supported by grants from the National Institutes of Health (HL-18575, GM-07767).

PY - 1999/11

Y1 - 1999/11

N2 - Experiments were designed to examine the role of sphingosine, PP2A phosphatases, and protein kinase C (PKC) inhibition in mediating the vasodilatory effects of ceramide in rat thoracic aorta. Sphingosine did not cause vasorelaxation, and oleoylethanolamine, a ceramidase inhibitor, did not affect sphingomyelinase-induced relaxation. Okadaic acid potentiated the relaxation response to ceramide. These observations rule out involvement of sphingosine and PP2A phosphatases in mediating ceramide-induced relaxation. Sphingomyelinase attenuated contractile and single-cell intracellular calcium responses to phorbol ester. Chelerythrine incubation potentiated the relaxation response to ceramide. These observations support a role for PKC inhibition in mediating the vasodilatory effects of ceramide. Copyright (C) 1999 Elsevier Science Inc.

AB - Experiments were designed to examine the role of sphingosine, PP2A phosphatases, and protein kinase C (PKC) inhibition in mediating the vasodilatory effects of ceramide in rat thoracic aorta. Sphingosine did not cause vasorelaxation, and oleoylethanolamine, a ceramidase inhibitor, did not affect sphingomyelinase-induced relaxation. Okadaic acid potentiated the relaxation response to ceramide. These observations rule out involvement of sphingosine and PP2A phosphatases in mediating ceramide-induced relaxation. Sphingomyelinase attenuated contractile and single-cell intracellular calcium responses to phorbol ester. Chelerythrine incubation potentiated the relaxation response to ceramide. These observations support a role for PKC inhibition in mediating the vasodilatory effects of ceramide. Copyright (C) 1999 Elsevier Science Inc.

KW - Ceramide

KW - Okadaic acid

KW - Oleoylethanolamine

KW - Sphingosine

KW - Vascular smooth muscle

KW - Vasorelaxation

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U2 - 10.1016/S0306-3623(99)00038-5

DO - 10.1016/S0306-3623(99)00038-5

M3 - Article

C2 - 10553883

AN - SCOPUS:0032726839

SN - 0306-3623

VL - 33

SP - 415

EP - 421

JO - General Pharmacology

JF - General Pharmacology

IS - 5

ER -

Ceramide-induced vasorelaxation: An inhibitory action on protein kinase C

Abstract

Keywords

ASJC Scopus subject areas

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