Changes in stiffness of demineralized dentin following application of collagen crosslinkers

Ana Karina B. Bedran-Russo, David H. Pashley, Kelli Agee, James L. Drummond, Klaus J. Miescke

Research output: Contribution to journalArticlepeer-review

186 Scopus citations


It is thought that increasing the strength of the dentin matrix using crosslinking agents may improve both the strength and the durability of resin-dentin bonds. The purpose of this study was to evaluate the effect of two collagen crosslinking agents (glutaraldehyde, GD and grape seed extract, GSE) on the modulus of elasticity of demineralized dentin. Sound molar fragments were fully demineralized and divided into five groups according to the type and concentration of crosslinking agents: 2.5% GD; 5% GD, 25% GD; 0.65% GSE; 6.5% GSE. Specimens were immersed in their respective solution and tested at baseline, 10 min, 30 min, 1 h, 2 h, 4 h. The elastic modulus of dentin was significantly affected by the treatment (p < 0.01) and exposure time (p < 0.01). There was a statistically significant interaction between the two factors evaluated (treatment vs. time p < 0.01). Mean baselines values varied between 4.8 and 6.2 MPa in water; after 4 h of treatment the values increased between 34.9 and 242.5 MPa, that were treatment time and agent dependent. The use of these collagen crosslinkers to increase the stiffness of demineralized dentin, was both concentration and time dependent.

Original languageEnglish (US)
Pages (from-to)330-334
Number of pages5
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number2
StatePublished - Aug 2008
Externally publishedYes


  • Collagen
  • Collagen cross-linking
  • Dental/craniofacial material
  • Modulus of elasticity
  • Surface modification

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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