Characterization of a late gene encoding for MCP in soft-shelled turtle iridovirus (STIV)

Zenglian Zhao; Yong Teng; Hong Liu; Xiangmei Lin; Kan Wang; Yulin Jiang; Huanchun Chen

doi:10.1016/j.virusres.2007.07.002

Characterization of a late gene encoding for MCP in soft-shelled turtle iridovirus (STIV)

Zenglian Zhao, Yong Teng, Hong Liu, Xiangmei Lin, Kan Wang, Yulin Jiang, Huanchun Chen

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Major caspid protein (MCP) is the major structural component of virus particles and revealed to be very responsible for classification of new tentative iridovirus isolates. In this paper, the complete sequences of MCP gene was firstly identified and characterized from soft-shelled turtle iridovirus (STIV). The MCP, classified as a late transcript by drug inhibition, encodes a protein of 463 aa with a predicted molecular weight of 50 kDa. Indirect immunofluorescence (IIF) and virus neutralization assay were developed to determine the sensitivity and virus neutralizing activity of MCP-specific antiserum. Furthermore, the MCP temporal expression pattern during STIV infection in vitro was characterized by Western blot and RT-PCR assays. The results suggest that STIV could be classified as a member of genus Ranavirus in family Iridoviridae and has cell-type-specific programs of viral gene expression.

Original language	English (US)
Pages (from-to)	135-144
Number of pages	10
Journal	Virus Research
Volume	129
Issue number	2
DOIs	https://doi.org/10.1016/j.virusres.2007.07.002
State	Published - Nov 2007
Externally published	Yes

Keywords

Late viral gene
Major caspid protein (MCP)
Ranavirus
Soft-shelled turtle iridovirus (STIV)
Temporal expression pattern

ASJC Scopus subject areas

Cancer Research
Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virusres.2007.07.002

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title = "Characterization of a late gene encoding for MCP in soft-shelled turtle iridovirus (STIV)",

abstract = "Major caspid protein (MCP) is the major structural component of virus particles and revealed to be very responsible for classification of new tentative iridovirus isolates. In this paper, the complete sequences of MCP gene was firstly identified and characterized from soft-shelled turtle iridovirus (STIV). The MCP, classified as a late transcript by drug inhibition, encodes a protein of 463 aa with a predicted molecular weight of 50 kDa. Indirect immunofluorescence (IIF) and virus neutralization assay were developed to determine the sensitivity and virus neutralizing activity of MCP-specific antiserum. Furthermore, the MCP temporal expression pattern during STIV infection in vitro was characterized by Western blot and RT-PCR assays. The results suggest that STIV could be classified as a member of genus Ranavirus in family Iridoviridae and has cell-type-specific programs of viral gene expression.",

keywords = "Late viral gene, Major caspid protein (MCP), Ranavirus, Soft-shelled turtle iridovirus (STIV), Temporal expression pattern",

author = "Zenglian Zhao and Yong Teng and Hong Liu and Xiangmei Lin and Kan Wang and Yulin Jiang and Huanchun Chen",

note = "Funding Information: The authors sincerely thank Dr. X.Y. Xie (Medical College of Georgia, USA) for critical comments and helpful discussion. This work was supported by the National Basic Research Program (973) of China (2005CB523200), China's General Administration for Quality Supervision, Inspection and Quarantine (AQSIQ) (2005IK034) and Institute of Hydrobiology of Chinese Academy of Sciences (2005FB14). ",

year = "2007",

month = nov,

doi = "10.1016/j.virusres.2007.07.002",

language = "English (US)",

volume = "129",

pages = "135--144",

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T1 - Characterization of a late gene encoding for MCP in soft-shelled turtle iridovirus (STIV)

AU - Zhao, Zenglian

AU - Teng, Yong

AU - Liu, Hong

AU - Lin, Xiangmei

AU - Wang, Kan

AU - Jiang, Yulin

AU - Chen, Huanchun

N1 - Funding Information: The authors sincerely thank Dr. X.Y. Xie (Medical College of Georgia, USA) for critical comments and helpful discussion. This work was supported by the National Basic Research Program (973) of China (2005CB523200), China's General Administration for Quality Supervision, Inspection and Quarantine (AQSIQ) (2005IK034) and Institute of Hydrobiology of Chinese Academy of Sciences (2005FB14).

PY - 2007/11

Y1 - 2007/11

N2 - Major caspid protein (MCP) is the major structural component of virus particles and revealed to be very responsible for classification of new tentative iridovirus isolates. In this paper, the complete sequences of MCP gene was firstly identified and characterized from soft-shelled turtle iridovirus (STIV). The MCP, classified as a late transcript by drug inhibition, encodes a protein of 463 aa with a predicted molecular weight of 50 kDa. Indirect immunofluorescence (IIF) and virus neutralization assay were developed to determine the sensitivity and virus neutralizing activity of MCP-specific antiserum. Furthermore, the MCP temporal expression pattern during STIV infection in vitro was characterized by Western blot and RT-PCR assays. The results suggest that STIV could be classified as a member of genus Ranavirus in family Iridoviridae and has cell-type-specific programs of viral gene expression.

AB - Major caspid protein (MCP) is the major structural component of virus particles and revealed to be very responsible for classification of new tentative iridovirus isolates. In this paper, the complete sequences of MCP gene was firstly identified and characterized from soft-shelled turtle iridovirus (STIV). The MCP, classified as a late transcript by drug inhibition, encodes a protein of 463 aa with a predicted molecular weight of 50 kDa. Indirect immunofluorescence (IIF) and virus neutralization assay were developed to determine the sensitivity and virus neutralizing activity of MCP-specific antiserum. Furthermore, the MCP temporal expression pattern during STIV infection in vitro was characterized by Western blot and RT-PCR assays. The results suggest that STIV could be classified as a member of genus Ranavirus in family Iridoviridae and has cell-type-specific programs of viral gene expression.

KW - Late viral gene

KW - Major caspid protein (MCP)

KW - Ranavirus

KW - Soft-shelled turtle iridovirus (STIV)

KW - Temporal expression pattern

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Characterization of a late gene encoding for MCP in soft-shelled turtle iridovirus (STIV)

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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